Comparative transcriptome profiling of a rice line carrying Xa39 and its parents triggered by Xanthomonas oryzae pv. oryzae provides novel insights into the broad-spectrum hypersensitive response

doi:10.1186/s12864-015-1329-3

	Comparative transcriptome profiling of a rice line carrying Xa39 and its parents triggered by Xanthomonas oryzae pv. oryzae provides novel insights into the broad-spectrum hypersensitive response
	Zhang, Fan 1; Huang, Li-Yu 1,2; Zhang, Fan 1; Ali, Jauhar 3; Cruz, Casiana Vera 3; Zhuo, Da-Long 1; Du, Zheng-Lin 4; Li, Zhi-kang 1; Zhou, Yong-Li 1
刊名	BMC GENOMICS
	2015
卷号	16 页码:-
关键词	Rice Xanthomonas oryzae pv. oryzae R gene Transcriptome sequencing
ISSN号	1471-2164
DOI	10.1186/s12864-015-1329-3
通讯作者	Zhang, Fan
英文摘要	Background: Bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is a devastating rice disease worldwide. Xa39 is a resistance (R) gene with a broad-spectrum hypersensitive response (BSHR) to Xoo. Nevertheless, the molecular mechanisms of resistance mediated by Xa39 remain unclear. In this study, the transcriptome profiling of a rice line carrying Xa39 and its parents at the early stage of Xoo infection were investigated.Results: A rice introgression line H471 carrying Xa39 exhibited a typical local hypersensitive response phenotype, accompanied by programmed cell death after inoculation with the Xoo Philippines' race 9b. Transcriptome profiling of H471 and its parents at 1 and 2 days post-inoculation was performed using RNA sequencing. In total, 306 differentially expressed genes (DEGs) were identified in H471 compared with its recurrent parent Huang-Hua-Zhan after inoculation with Xoo. Among them, 121 (39.5%) genes, with functional enrichments that were related to defense response, protein amino acid phosphorylation, and apoptosis, were found to be constitutively expressed. The other 185 (60.5%) genes, with GO terms that belonged to defense response, were significantly responsive to Xoo infection in H471. Ten up-regulated and 12 down-regulated genes encoding intracellular immune receptors were identified in H471 compared with Huang-Hua-Zhan. LOC_Os11g37759, which was located in the fine-mapping region harboring Xa39, is a Xa39 candidate gene. The putative BSHR-related co-regulatory networks were constructed using 33 DEGs from four functional groups, including gibberellic acid receptors and brassinosteroid regulators, which were differentially co-expressed with LOC_Os11g37759 in infected H471. Our results indicated that there might be cross-talk between the Xa39-mediated signal transduction cascades and the GA/BR signaling pathway, and that the defense mechanism was related to diverse kinases, transcription factors, post-translational regulation, and R genes.Conclusions: The present study provides the comprehensive transcriptome profile of a rice introgression line carrying Xa39 and its parents, and identifies a set of DEGs involved in BSHR mediated by Xa39. These data provide novel insights into the regulatory networks of plant disease resistance mediated by R genes, and the identified DEGs will serve as candidates for Xa39 cloning and for further understanding the molecular mechanism of BSHR.
学科主题	Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000350381100001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4762]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Yunnan Acad Agr Sci, Food Crops Res Inst, Kunming 650250, Yunnan, Peoples R China 3.Int Rice Res Inst, Manila, Philippines 4.Chinese Acad Sci, Beijing Inst Gen, Beijing 100029, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Fan,Huang, Li-Yu,Zhang, Fan,et al. Comparative transcriptome profiling of a rice line carrying Xa39 and its parents triggered by Xanthomonas oryzae pv. oryzae provides novel insights into the broad-spectrum hypersensitive response[J]. BMC GENOMICS,2015,16:-.
APA	Zhang, Fan.,Huang, Li-Yu.,Zhang, Fan.,Ali, Jauhar.,Cruz, Casiana Vera.,...&Zhou, Yong-Li.(2015).Comparative transcriptome profiling of a rice line carrying Xa39 and its parents triggered by Xanthomonas oryzae pv. oryzae provides novel insights into the broad-spectrum hypersensitive response.BMC GENOMICS,16,-.
MLA	Zhang, Fan,et al."Comparative transcriptome profiling of a rice line carrying Xa39 and its parents triggered by Xanthomonas oryzae pv. oryzae provides novel insights into the broad-spectrum hypersensitive response".BMC GENOMICS 16(2015):-.