Two alternative splicing variants of maize HKT1;1 confer salt tolerance in transgenic tobacco plants

doi:10.1007/s11240-015-0861-9

	Two alternative splicing variants of maize HKT1;1 confer salt tolerance in transgenic tobacco plants
	Ren, Zhenjing 1; Liu, Yan 2; Kang, Dan 1; Fan, Kaijian 1; Wang, Cuiyun 1; Wang, Guoying 1; Liu, Yunjun 1
刊名	PLANT CELL TISSUE AND ORGAN CULTURE
	2015
卷号	123 期号:3 页码:569-578
关键词	Maize HKT Alternative splicing Salt tolerance Transgenic plant
ISSN号	0167-6857
DOI	10.1007/s11240-015-0861-9
通讯作者	Ren, Zhenjing
英文摘要	The high-affinity potassium transporter (HKT) genes have crucial roles in the regulation of sodium and potassium transportation in many species, however little is known about maize HKT genes. In this study, we obtained two alternative splicing transcripts of ZmHKT1;1 gene. One was named as ZmHKT1;1a which has the intact coding sequence, and the other was named as ZmHKT1;1b which has a deficiency of the third exon and a retention of the second intron. The phylogenic tree analysis showed that both translation products of ZmHKT1;1a and ZmHKT1;1b belong to group I HKT proteins which prefer for Na+ transport than other cations. ZmHKT1;1a and ZmHKT1;1b showed different response to stress treatment in maize. Overexpressing ZmHKT1;1a or ZmHKT1;1b in transgenic tobacco plants conferred high salt tolerance by increasing root length and fresh weight of plants. When treated with high concentration of salt, transgenic tobacco plants manifested a trend of reduced Na+ content and increased K+ content in both shoot and root, suggesting that ZmHKT1;1 may involve in Na+ unloading and indirectly affect other transporter activity. It was also found that overexpression of ZmHKT1;1a and ZmHKT1;1b caused different expression of stress-related genes. The results in this study indicate that two alternative splicing variants of ZmHKT1;1 might be useful for the development of salt-tolerant transgenic crops.
学科主题	Biotechnology & Applied Microbiology ; Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000365216300012
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4622]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 2.China Agr Univ, Coll Agr & Biotechnol, Beijing 100094, Peoples R China
推荐引用方式 GB/T 7714	Ren, Zhenjing,Liu, Yan,Kang, Dan,et al. Two alternative splicing variants of maize HKT1;1 confer salt tolerance in transgenic tobacco plants[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2015,123(3):569-578.
APA	Ren, Zhenjing.,Liu, Yan.,Kang, Dan.,Fan, Kaijian.,Wang, Cuiyun.,...&Liu, Yunjun.(2015).Two alternative splicing variants of maize HKT1;1 confer salt tolerance in transgenic tobacco plants.PLANT CELL TISSUE AND ORGAN CULTURE,123(3),569-578.
MLA	Ren, Zhenjing,et al."Two alternative splicing variants of maize HKT1;1 confer salt tolerance in transgenic tobacco plants".PLANT CELL TISSUE AND ORGAN CULTURE 123.3(2015):569-578.