Mechanisms of Soybean Roots' Tolerances to Salinity Revealed by Proteomic and Phosphoproteomic Comparisons Between Two Cultivars

doi:10.1074/mcp.M115.051961

	Mechanisms of Soybean Roots' Tolerances to Salinity Revealed by Proteomic and Phosphoproteomic Comparisons Between Two Cultivars
	Pi, Erxu 1; Qu, Liqun 1; Hu, Jianwen 2; Huang, Yingying 1; Qiu, Lijuan 3; Lu, Hongfei 4; Jiang, Bo 5; Liu, Cong 1; Peng, Tingting 1; Zhao, Ying 1
刊名	MOLECULAR & CELLULAR PROTEOMICS
	2016
卷号	15 期号:1 页码:266-288
ISSN号	1535-9476
DOI	10.1074/mcp.M115.051961
通讯作者	Pi, Erxu
英文摘要	Understanding molecular mechanisms underlying plant salinity tolerance provides valuable knowledgebase for effective crop improvement through genetic engineering. Current proteomic technologies, which support reliable and high-throughput analyses, have been broadly used for exploring sophisticated molecular networks in plants. In the current study, we compared phosphoproteomic and proteomic changes in roots of different soybean seedlings of a salt-tolerant cultivar (Wenfeng07) and a salt-sensitive cultivar (Union85140) induced by salt stress. The root samples of Wenfeng07 and Union85140 at three-trifoliate stage were collected at 0 h, 0.5 h, 1 h, 4 h, 12 h, 24 h, and 48 h after been treated with 150 mM NaCl. LC-MS/MS based phosphoproteomic analysis of these samples identified a total of 2692 phosphoproteins and 5509 phosphorylation sites. Of these, 2344 phosphoproteins containing 3744 phosphorylation sites were quantitatively analyzed. Our results showed that 1163 phosphorylation sites were differentially phosphorylated in the two compared cultivars. Among them, 10 MYB/MYB transcription factor like proteins were identified with fluctuating phosphorylation modifications at different time points, indicating that their crucial roles in regulating flavonol accumulation might be mediated by phosphorylated modifications. In addition, the protein expression profiles of these two cultivars were compared using LC-MS/MS based shotgun proteomic analysis, and expression pattern of all the 89 differentially expressed proteins were independently confirmed by qRT-PCR. Interestingly, the enzymes involved in chalcone metabolic pathway exhibited positive correlations with salt tolerance. We confirmed the functional relevance of chalcone synthase, chalcone isomerase, and cytochrome P450 monooxygenase genes using soybean composites and Arabidopsis thaliana mutants, and found that their salt tolerance were positively regulated by chalcone synthase, but was negatively regulated by chalcone isomerase and cytochrome P450 monooxygenase. A novel salt tolerance pathway involving chalcone metabolism, mostly mediated by phosphorylated MYB transcription factors, was proposed based on our findings. (The mass spectrometry raw data are available via ProteomeXchange with identifier PXD002856).
学科主题	Biochemical Research Methods
语种	英语
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
WOS记录号	WOS:000367461000019
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4592]
专题	作物科学研究所_分子生物学系
作者单位	1.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 310036, Zhejiang, Peoples R China 2.Shanghai Appl Prot Technol Co Ltd, Shanghai 200233, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100193, Peoples R China 4.Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310018, Zhejiang, Peoples R China 5.Changshu Inst Technol, Coll Biol & Food Engn, Changshu 215500, Peoples R China 6.Chinese Univ Hong Kong, Ctr Soybean Res, Partner State Key Lab Agrobiotechnol, Hong Kong, Hong Kong, Peoples R China 7.Chinese Univ Hong Kong, Sch Life Sci, Hong Kong, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Pi, Erxu,Qu, Liqun,Hu, Jianwen,et al. Mechanisms of Soybean Roots' Tolerances to Salinity Revealed by Proteomic and Phosphoproteomic Comparisons Between Two Cultivars[J]. MOLECULAR & CELLULAR PROTEOMICS,2016,15(1):266-288.
APA	Pi, Erxu.,Qu, Liqun.,Hu, Jianwen.,Huang, Yingying.,Qiu, Lijuan.,...&Du, Liqun.(2016).Mechanisms of Soybean Roots' Tolerances to Salinity Revealed by Proteomic and Phosphoproteomic Comparisons Between Two Cultivars.MOLECULAR & CELLULAR PROTEOMICS,15(1),266-288.
MLA	Pi, Erxu,et al."Mechanisms of Soybean Roots' Tolerances to Salinity Revealed by Proteomic and Phosphoproteomic Comparisons Between Two Cultivars".MOLECULAR & CELLULAR PROTEOMICS 15.1(2016):266-288.