Wax crystal-sparse leaf 3 encoding a beta-ketoacyl-CoA reductase is involved in cuticular wax biosynthesis in rice

doi:10.1007/s00299-016-1983-1

	Wax crystal-sparse leaf 3 encoding a beta-ketoacyl-CoA reductase is involved in cuticular wax biosynthesis in rice
	Gan, Lu 1; Wang, Xiaole 1; Cheng, Zhijun 1; Liu, Linglong 2; Wang, Jiulin 1; Zhang, Zhe 1; Ren, Yulong 1; Lei, Cailin 1; Zhao, Zhichao 1; Zhu, Shanshan 1
刊名	PLANT CELL REPORTS
	2016
卷号	35 期号:8 页码:1687-1698
关键词	Wax crystal-sparse leaf 3 beta-Ketoacyl-CoA reductase Cuticular wax Fatty acid elongase Rice (Oryza sativa)
ISSN号	0721-7714
DOI	10.1007/s00299-016-1983-1
通讯作者	Gan, Lu
英文摘要	WSL3 encodes beta-ketoacyl-CoA reductase (KCR) in rice, in a similar way to YBR159w in yeast, and is essential for VLCFA biosynthesis and leaf wax accumulation.Cuticular waxes on plant surfaces limit non-stomatal water loss, protect plants against deposits of dust and impose a physical barrier to pathogen infection. We identified a wax-deficient mutant of rice, wax crystal-sparse leaf 3 (wsl3), which exhibits a pleiotropic phenotype that includes reduced epicuticular wax crystals on the leaf surface and altered wax composition. Map-based cloning demonstrated that defects in the mutant were caused by two adjacent single-nucleotide changes in a gene encoding beta-ketoacyl-CoA reductase (KCR) that catalyzes the second step of the fatty acid elongation reaction. The identity of WSL3 was further confirmed by genetic complementation. Transient assays of fluorescent protein-tagged WSL3 in tobacco protoplasts showed that WSL3 localizes to the endoplasmic reticulum, the compartment of fatty acid elongation in cells. Quantitative PCR and histochemical staining indicated that WSL3 is universally expressed in tissues. RNA interference of WSL3 caused a phenotype that mimicked the wsl3 mutant. Very long-chain fatty acids (VLCFAs) 20:0 and 22:0, or 20:1 Delta(11) and 22:1 Delta(13), were detected when WSL3 and Arabidopsis fatty acid elongation 1 (FAE1) were co-expressed in a yeast ybr159w Delta mutant strain. Our results indicated that WSL3 affects rice cuticular wax production by participating in VLCFA elongation.
学科主题	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000379851100009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4485]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Nanjing Agr Univ, Jiangsu Plant Gene Engn Res Ctr, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Gan, Lu,Wang, Xiaole,Cheng, Zhijun,et al. Wax crystal-sparse leaf 3 encoding a beta-ketoacyl-CoA reductase is involved in cuticular wax biosynthesis in rice[J]. PLANT CELL REPORTS,2016,35(8):1687-1698.
APA	Gan, Lu.,Wang, Xiaole.,Cheng, Zhijun.,Liu, Linglong.,Wang, Jiulin.,...&Wan, Jianmin.(2016).Wax crystal-sparse leaf 3 encoding a beta-ketoacyl-CoA reductase is involved in cuticular wax biosynthesis in rice.PLANT CELL REPORTS,35(8),1687-1698.
MLA	Gan, Lu,et al."Wax crystal-sparse leaf 3 encoding a beta-ketoacyl-CoA reductase is involved in cuticular wax biosynthesis in rice".PLANT CELL REPORTS 35.8(2016):1687-1698.