Allelic Variants at the Psy-A1 and Psy-B1 Loci in Durum Wheat and Their Associations with Grain Yellowness

doi:10.2135/cropsci2008.11.0651

	Allelic Variants at the Psy-A1 and Psy-B1 Loci in Durum Wheat and Their Associations with Grain Yellowness
	He, Xinyao 1; Wang, Jianwu 1,2; Ammar, Karim 4; Pena, Roberto Javier 4; Xia, Xianchun 1; He, Zhonghu 1,3
刊名	CROP SCIENCE
	2009
卷号	49 期号:6 页码:2058-2064
ISSN号	0011-183X
DOI	10.2135/cropsci2008.11.0651
通讯作者	He, Xinyao
英文摘要	Phytoene synthase (PSY) genes are involved in the biosynthesis of carotenoid pigments in durum wheat [Triticum turgidum L. subsp. durum (Desf.) Husn.], significantly influencing grain yellowness. This study was conducted to identify new allelic variants at the Psy-A1 and Psy-B1 loci in durum wheat, and to evaluate the applicability of functional markers developed from common wheat (Triticum aestivum L.) for durum wheat breeding. Two new allelic variants, Psy-A1d and Psy-A1e, were identified at the Psy-A1 locus, and both the codominant markers YP7A and YP7A-2 can be used to discriminate the two haplotypes, yielding 194- and 231-bp polymerase chain reaction (PCR) products with YP7A and 1001- and 1684-bp fragments with YP7A-2, respectively. At the Psy-B1 locus, three allelic variants were identified. Psy-B1e was also found in common wheat, whereas Psy-B1f and Psy-B1g were detected only in durum wheat. The codominant marker YP7B-1 can be used to distinguish Psy-B1f and Psy-B1g, generating 151- and 153-bp PCR fragments, respectively, and the dominant marker YP7B-4 was specific to haplotype Psy-B1e, producing a 717-bp PCR product. In a set of 100 CIMMYT durum wheat lines with widely variable grain yellowness, the frequencies of Psy-A1d, Psy-A1e, Psy-B1e, Psy-B1f, and Psy-B1g were 99, 1, 0, 67, and 33%, respectively, and the genotype Psy-B1f showed a significant association with higher grain yellowness, whereas the presence of Psy-B1g led to lower yellowness. A phylogenetic tree generated from the gene sequences of the allelic variants at Psy-A1 and Psy-B1 loci indicated two parallel lineages of durum-common wheat, suggesting that more than one tetraploid T. turgidum L. subsp. dicoccon (Schrank) Thell. genotypes were involved in the origin of common wheat. Our results suggested that Psy-B1f should be paid more attention in durum breeding programs for its association with elevated grain yellowness.
学科主题	Agronomy ; AGRONOMY
语种	英语
出版者	CROP SCIENCE SOC AMER
WOS记录号	WOS:000271607300013
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5480]
专题	作物科学研究所_遗传育种系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.NW A&F Univ, Coll Agron, Yangling 712100, Shaanxi, Peoples R China 3.CAAS, China Off, Int Maize & Wheat Improvement Ctr CIMMYT, Beijing 100081, Peoples R China 4.CIMMYT, Int Maize & Wheat Improvement Ctr, Mexico City 06600, DF, Mexico
推荐引用方式 GB/T 7714	He, Xinyao,Wang, Jianwu,Ammar, Karim,et al. Allelic Variants at the Psy-A1 and Psy-B1 Loci in Durum Wheat and Their Associations with Grain Yellowness[J]. CROP SCIENCE,2009,49(6):2058-2064.
APA	He, Xinyao,Wang, Jianwu,Ammar, Karim,Pena, Roberto Javier,Xia, Xianchun,&He, Zhonghu.(2009).Allelic Variants at the Psy-A1 and Psy-B1 Loci in Durum Wheat and Their Associations with Grain Yellowness.CROP SCIENCE,49(6),2058-2064.
MLA	He, Xinyao,et al."Allelic Variants at the Psy-A1 and Psy-B1 Loci in Durum Wheat and Their Associations with Grain Yellowness".CROP SCIENCE 49.6(2009):2058-2064.