Alternative splicing in the coding region of Ppo-A1 directly influences the polyphenol oxidase activity in common wheat (Triticum aestivum L.)

doi:10.1007/s10142-010-0201-4

	Alternative splicing in the coding region of Ppo-A1 directly influences the polyphenol oxidase activity in common wheat (Triticum aestivum L.)
	Sun, Youwei 1; He, Zhonghu 1,2; Ma, Wujun 3; Xia, Xianchun 1
刊名	FUNCTIONAL & INTEGRATIVE GENOMICS
	2011
卷号	11 期号:1 页码:85-93
关键词	Alternative splicing Nonsense-mediated mRNA decay Premature termination codon PPO gene
ISSN号	1438-793X
DOI	10.1007/s10142-010-0201-4
通讯作者	Sun, Youwei
英文摘要	Polyphenol oxidase (PPO) plays a crucial role in browning reactions in fresh and processed fruits and vegetables, as well as products made from cereal grains. Common wheat (Triticum aestivum L.) has a large genome, representing an interesting system to advance our understanding of plant PPO gene expression, regulation and function. In the present study, we characterized the expression of Ppo-A1, a major PPO gene located on wheat chromosome 2A, using DNA sequencing, semi-quantitative RT-PCR, PPO activity assays and whole-grain staining methods during grain development. The results indicated that the expression of the Ppo-A1b allele was regulated by alternative splicing of pre-mRNAs, resulting from a 191-bp insertion in intron 1 and one C/G SNP in exon 2. Eight mRNA isoforms were identified in developing grains based on alignments between cDNA and genomic DNA sequences. Only the constitutively spliced isoform b encodes a putative full-length PPO protein based on its coding sequence whereas the other seven spliced isoforms, a, c, d, e, f, g and h, have premature termination codons resulting in potential nonsense-mediated mRNA decay. The differences in expression of Ppo-A1a and Ppo-A1b were confirmed by PPO activity assays and whole grain staining, providing direct evidence for the influence of alternative splicing in the coding region of Ppo-A1 on polyphenol oxidase activity in common wheat grains.
学科主题	Genetics & Heredity ; GENETICS & HEREDITY
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000289725000008
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5337]
专题	作物科学研究所_遗传育种系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.CAAS, China Off, Int Maize & Wheat Improvement Ctr CIMMYT, Beijing 100081, Peoples R China 3.Murdoch Univ, Dept Agr & Food, Western Australia Ctr Comparat Genom, Perth, WA 6150, Australia
推荐引用方式 GB/T 7714	Sun, Youwei,He, Zhonghu,Ma, Wujun,et al. Alternative splicing in the coding region of Ppo-A1 directly influences the polyphenol oxidase activity in common wheat (Triticum aestivum L.)[J]. FUNCTIONAL & INTEGRATIVE GENOMICS,2011,11(1):85-93.
APA	Sun, Youwei,He, Zhonghu,Ma, Wujun,&Xia, Xianchun.(2011).Alternative splicing in the coding region of Ppo-A1 directly influences the polyphenol oxidase activity in common wheat (Triticum aestivum L.).FUNCTIONAL & INTEGRATIVE GENOMICS,11(1),85-93.
MLA	Sun, Youwei,et al."Alternative splicing in the coding region of Ppo-A1 directly influences the polyphenol oxidase activity in common wheat (Triticum aestivum L.)".FUNCTIONAL & INTEGRATIVE GENOMICS 11.1(2011):85-93.