Genome-Wide Identification of MicroRNAs in Response to Low Nitrate Availability in Maize Leaves and Roots

doi:10.1371/journal.pone.0028009

	Genome-Wide Identification of MicroRNAs in Response to Low Nitrate Availability in Maize Leaves and Roots
	Xu, Zhenhua 1; Zhong, Sihui 2; Li, Xinhai 1; Li, Wenxue 1; Rothstein, Steven J.2; Zhang, Shihuang 1; Bi, Yongmei 2; Xie, Chuanxiao 1
刊名	PLOS ONE
	2011
卷号	6 期号:11 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0028009
通讯作者	Xu, Zhenhua
英文摘要	Background: Nitrate is the major source of nitrogen available for many crop plants and is often the limiting factor for plant growth and agricultural productivity especially for maize. Many studies have been done identifying the transcriptome changes under low nitrate conditions. However, the microRNAs (miRNAs) varied under nitrate limiting conditions in maize has not been reported. MiRNAs play important roles in abiotic stress responses and nutrient deprivation.Methodology/ Principal Findings: In this study, we used the SmartArray (TM) and GeneChip (R) microarray systems to perform a genome-wide search to detect miRNAs responding to the chronic and transient nitrate limiting conditions in maize. Nine miRNA families (miR164, miR169, miR172, miR397, miR398, miR399, miR408, miR528, and miR827) were identified in leaves, and nine miRNA families (miR160, miR167, miR168, miR169, miR319, miR395, miR399, miR408, and miR528) identified in roots. They were verified by real time stem loop RT-PCR, and some with additional time points of nitrate limitation. The miRNAs identified showed overlapping or unique responses to chronic and transient nitrate limitation, as well as tissue specificity. The potential target genes of these miRNAs in maize were identified. The expression of some of these was examined by qRT-PCR. The potential function of these miRNAs in responding to nitrate limitation is described.Conclusions/Significance: Genome-wide miRNAs responding to nitrate limiting conditions in maize leaves and roots were identified. This provides an insight into the timing and tissue specificity of the transcriptional regulation to low nitrate availability in maize. The knowledge gained will help understand the important roles miRNAs play in maize responding to a nitrogen limiting environment and eventually develop strategies for the improvement of maize genetics.
学科主题	Multidisciplinary Sciences ; MULTIDISCIPLINARY SCIENCES
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000298162000058
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5251]
专题	作物科学研究所_遗传育种系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100193, Peoples R China 2.Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON N1G 2W1, Canada
推荐引用方式 GB/T 7714	Xu, Zhenhua,Zhong, Sihui,Li, Xinhai,et al. Genome-Wide Identification of MicroRNAs in Response to Low Nitrate Availability in Maize Leaves and Roots[J]. PLOS ONE,2011,6(11):-.
APA	Xu, Zhenhua.,Zhong, Sihui.,Li, Xinhai.,Li, Wenxue.,Rothstein, Steven J..,...&Xie, Chuanxiao.(2011).Genome-Wide Identification of MicroRNAs in Response to Low Nitrate Availability in Maize Leaves and Roots.PLOS ONE,6(11),-.
MLA	Xu, Zhenhua,et al."Genome-Wide Identification of MicroRNAs in Response to Low Nitrate Availability in Maize Leaves and Roots".PLOS ONE 6.11(2011):-.