Functional Analysis of GmCPDs and Investigation of Their Roles in Flowering

doi:10.1371/journal.pone.0118476

	Functional Analysis of GmCPDs and Investigation of Their Roles in Flowering
	Wang, Miao 1,2; Xu, Xin 1; Zhang, Xinxin 1; Sun, Shi 1; Wu, Cunxiang 1; Hou, Wensheng 1; Wang, Qingyu 2; Han, Tianfu 1
刊名	PLOS ONE
	2015
卷号	10 期号:3 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0118476
通讯作者	Wang, Miao
英文摘要	The onset of floral development is a pivotal switch in the life of soybean. Brassinosteroids (BRs), a group of steroidal phytohormones with essential roles in plant growth and development, are associated with flowering induction. Genes involved in BR biosynthesis have been studied to a great extent in Arabidopsis, but the study of these genes has been limited in soybean. In this study, four CPD homologs (GmCPDs) catalyzing BR synthesis were isolated from soybean. Transcripts were mainly confined to cotyledons and leaves and were down-regulated in response to exogenous BR. Bioinformatic analysis showed strong sequence and structure similarity between GmCPDs and AtCPD as well as CPDs of other species. Overexpression of GmCPDs in an Arabidopsis BR-deficient mutant rescued the phenotype by restoring the biosynthesis pathway, revealing the functional roles of each GmCPDs in. Except for the rescue of root development, leaf expansion and plant type architecture, GmCPDs in expression also complemented the late flowering phenotype of Arabidopsis mutants deficient in CPD. Further evidence in soybean plants is that the expression levels of GmCPDs in are under photoperiod control in Zigongdongdou, a photoperiod-sensitive variety, and show a sudden peak upon floral meristem initiation. Together with increased GmCPDs in expression in the leaves and cotyledons of photoperiod- insensitive early-maturity soybean, it is clear that GmCPDs in contribute to flowering development and are essential in the early stages of flowering regulation.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000350314700032
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4746]
专题	作物科学研究所_遗传育种系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Minist Agr, Key Lab Soybean Biol Beijing, Beijing 100081, Peoples R China 2.Jilin Univ, Coll Plant Sci, Changchun 130062, Jilin, Peoples R China
推荐引用方式 GB/T 7714	Wang, Miao,Xu, Xin,Zhang, Xinxin,et al. Functional Analysis of GmCPDs and Investigation of Their Roles in Flowering[J]. PLOS ONE,2015,10(3):-.
APA	Wang, Miao.,Xu, Xin.,Zhang, Xinxin.,Sun, Shi.,Wu, Cunxiang.,...&Han, Tianfu.(2015).Functional Analysis of GmCPDs and Investigation of Their Roles in Flowering.PLOS ONE,10(3),-.
MLA	Wang, Miao,et al."Functional Analysis of GmCPDs and Investigation of Their Roles in Flowering".PLOS ONE 10.3(2015):-.