A high-density genetic map for P genome of Agropyron Gaertn. based on specific-locus amplified fragment sequencing (SLAF-seq)

doi:10.1007/s00425-015-2372-7

	A high-density genetic map for P genome of Agropyron Gaertn. based on specific-locus amplified fragment sequencing (SLAF-seq)
	Zhang, Yan 1,2; Zhang, Jinpeng 1; Huang, Long 3; Gao, Ainong 1; Zhang, Jing 1; Yang, Xinming 1; Liu, Weihua 1; Li, Xiuquan 1; Li, Lihui 1
刊名	PLANTA
	2015
卷号	242 期号:6 页码:1335-1347
关键词	Agropyron Genetic map High density SLAF marker
ISSN号	0032-0935
DOI	10.1007/s00425-015-2372-7
通讯作者	Zhang, Yan
英文摘要	This genetic map for Agropyron Gaertn. contained 1023 markers on seven linkage groups, with a total of 907.8 cM and an average distance of 1.5 cM between adjacent loci.Many wheat- Agropyron cristatum derivative lines exhibit superior agronomic traits, and part of them are valuable for future wheat breeding. To date, no high-density genetic map for Agropyron Gaertn. has been published. Specific-locus amplified fragment sequencing (SLAF-seq), a recently developed strategy for large scale de novo discovery and genotyping of single nucleotide polymorphisms (SNPs), was employed in this study to develop sufficient markers for a segregating Agropyron F-1 population derived from an interspecific cross between two cross-pollinated diploid collections A. cristatum (L.) Beauv. 'Z1842' and A. mongolicum Keng 'Z2098'. In total, we obtained raw data consisting of 128,932,358 pair-end reads of 80 bp long after sequencing. Then 69,325 high-quality SLAFs were detected, of which 26,248 SLAFs were polymorphic and 1752 of the polymorphic markers were used for the genetic map construction. The final map contained 1023 markers on the seven linkage groups (LGs), which spanned a total of 907.8 cM with an average number of 146 markers and 89 loci per LG and an average distance of 1.5 cM between adjacent loci. To our knowledge, this map is the densest genetic linkage map for Agropyron so far. Through BLAT alignment of Agropyron SLAF marker sequences with the draft genome assemblies of wheat and barley, the Agropyron LGs were assigned as LG1-7 according to their corresponding homoeologous chromosomal groups of wheat. Results of this study will not only provide a platform for gene/QTL fine mapping, but also serve as a reference to assist the assembling of the P genome sequence in future.
学科主题	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000363042600006
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4621]
专题	作物科学研究所_遗传育种系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Chinese Acad Agr Sci, Biotechnol Res Inst, Dept Crop Genom & Genet Improvement, Beijing 100081, Peoples R China 3.Biomarker Technol Corp, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yan,Zhang, Jinpeng,Huang, Long,et al. A high-density genetic map for P genome of Agropyron Gaertn. based on specific-locus amplified fragment sequencing (SLAF-seq)[J]. PLANTA,2015,242(6):1335-1347.
APA	Zhang, Yan.,Zhang, Jinpeng.,Huang, Long.,Gao, Ainong.,Zhang, Jing.,...&Li, Lihui.(2015).A high-density genetic map for P genome of Agropyron Gaertn. based on specific-locus amplified fragment sequencing (SLAF-seq).PLANTA,242(6),1335-1347.
MLA	Zhang, Yan,et al."A high-density genetic map for P genome of Agropyron Gaertn. based on specific-locus amplified fragment sequencing (SLAF-seq)".PLANTA 242.6(2015):1335-1347.