Development and genetic mapping of SSR markers in foxtail millet [Setaria italica (L.) P. Beauv.]

doi:10.1007/s00122-008-0942-9

	Development and genetic mapping of SSR markers in foxtail millet [Setaria italica (L.) P. Beauv.]
	Jia, Xiaoping; Zhang, Zhongbao; Liu, Yinghui; Zhang, Chengwei; Shi, Yunsu; Song, Yanchun; Wang, Tianyu; Li, Yu
刊名	THEORETICAL AND APPLIED GENETICS
	2009
卷号	118 期号:4 页码:821-829
ISSN号	0040-5752
DOI	10.1007/s00122-008-0942-9
通讯作者	Jia, Xiaoping
英文摘要	SSR markers are desirable markers in analysis of genetic diversity, quantitative trait loci mapping and gene locating. In this study, SSR markers were developed from two genomic libraries enriched for (GA)n and (CA)n of foxtail millet [Setaria italica (L.) P. Beauv.], a crop of historical importance in China. A total of 100 SSR markers among the 193 primer pairs detected polymorphism between two mapping parents of an F(2) population, i.e. "B100" of cultivated S. italica and "A10" of wild S. viridis. Excluding 14 markers with unclear amplifications, and five markers unlinked with any linkage group, a foxtail millet SSR linkage map was constructed by integrating 81 new developed SSR markers with 20 RFLP anchored markers. The 81 SSRs covered nine chromosomes of foxtail millet. The length of the map was 1,654 cM, with an average interval distance between markers of 16.4 cM. The 81 SSR markers were not evenly distributed throughout the nine chromosomes, with Ch.8 harbouring the least (3 markers) and Ch.9 harbouring the most (18 markers). To verify the usefulness of the SSR markers developed, 37 SSR markers were randomly chosen to analyze genetic diversity of 40 foxtail millet accessions. Totally 228 alleles were detected, with an average 6.16 alleles per locus. Polymorphism information content (PIC) value for each locus ranged from 0.413 to 0.847, with an average of 0.697. A positive correlation between PIC and number of alleles and between PIC and number of repeat unit were found [0.802 and 0.429, respectively (P < 0.01)]. UPGMA analysis revealed that the 40 foxtail millet cultivars could be grouped into five clusters in which the landraces' grouping was largely consistent with ecotypes while the breeding varieties from different provinces in China tended to be grouped together.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000263391800018
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5537]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, NFCRI, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Jia, Xiaoping,Zhang, Zhongbao,Liu, Yinghui,et al. Development and genetic mapping of SSR markers in foxtail millet [Setaria italica (L.) P. Beauv.][J]. THEORETICAL AND APPLIED GENETICS,2009,118(4):821-829.
APA	Jia, Xiaoping.,Zhang, Zhongbao.,Liu, Yinghui.,Zhang, Chengwei.,Shi, Yunsu.,...&Li, Yu.(2009).Development and genetic mapping of SSR markers in foxtail millet [Setaria italica (L.) P. Beauv.].THEORETICAL AND APPLIED GENETICS,118(4),821-829.
MLA	Jia, Xiaoping,et al."Development and genetic mapping of SSR markers in foxtail millet [Setaria italica (L.) P. Beauv.]".THEORETICAL AND APPLIED GENETICS 118.4(2009):821-829.