Fine mapping and candidate gene analysis of the floury endosperm gene, FLO(a), in rice

doi:10.1007/s10059-010-0010-6

	Fine mapping and candidate gene analysis of the floury endosperm gene, FLO(a), in rice
	Qiao, Yongli 1,2; Lee, Song-I 1,2; Piao, Rihua 1,2; Jiang, Wenzhu 1,2; Ham, Tae-Ho 1,2; Chin, Joong-Hyoun 3; Piao, Zhongze 4; Han, Longzhi 5; Kang, Si-Yong 6; Koh, Hee-Jong 1,2
刊名	MOLECULES AND CELLS
	2010
卷号	29 期号:2 页码:167-174
关键词	floury endosperm mapping rice starch tetratricopeptide repeat domain-containing protein
ISSN号	1016-8478
DOI	10.1007/s10059-010-0010-6
通讯作者	Qiao, Yongli
英文摘要	In addition to its role as an energy source for plants, animals and humans, starch is also an environmentally friendly alternative to fossil fuels. In rice, the eating and cooking quality of the grain is determined by its starch properties. The floury endosperm of rice has been explored as an agronomical trait in breeding and genetics studies. In the present study, we characterized a floury endosperm mutant, flo(a), derived from treatment of Oryza sativa ssp. japonica cultivar Hwacheong with MNU. The innermost endosperm of the flo(a) mutant exhibited floury characteristics while the outer layer of the endosperm appeared normal. Starch granules in the flo(a) mutant formed a loosely-packed crystalline structure and X-ray diffraction revealed that the overall crystallinity of the starch was decreased compared to wild-type. The FLO(a) gene was isolated via a map-based cloning approach and predicted to encode the tetratricopeptide repeat domaincontaining protein, OsTPR. Three mutant alleles contain a nucleotide substitution that generated one stop codon or one splice site, respectively, which presumably disrupts the interaction of the functionally conserved TPR motifs. Taken together, our map-based cloning approach pinpointed an OsTPR as a strong candidate of FLO(a), and the proteins that contain TPR motifs might play a significant role in rice starch biosynthetic pathways.
学科主题	Biochemistry & Molecular Biology ; Cell Biology ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; CELL BIOLOGY
语种	英语
出版者	KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
WOS记录号	WOS:000275123100009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5450]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.Seoul Natl Univ, Dept Plant Sci, Res Inst Agr & Life Sci, Seoul 151921, South Korea 2.Seoul Natl Univ, Plant Genom & Breeding Inst, Seoul 151921, South Korea 3.Int Rice Res Inst, Manila 1099, Philippines 4.Shanghai Acad Agr Sci, Crop Res Inst, Shanghai 201106, Peoples R China 5.Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Crop Germplasm Resources & Biotechnol, Minist Agr, Beijing 100081, Peoples R China 6.Korea Atom Energy Res Inst, Adv Radiat Technol Inst, Jungeub 580910, South Korea
推荐引用方式 GB/T 7714	Qiao, Yongli,Lee, Song-I,Piao, Rihua,et al. Fine mapping and candidate gene analysis of the floury endosperm gene, FLO(a), in rice[J]. MOLECULES AND CELLS,2010,29(2):167-174.
APA	Qiao, Yongli.,Lee, Song-I.,Piao, Rihua.,Jiang, Wenzhu.,Ham, Tae-Ho.,...&Koh, Hee-Jong.(2010).Fine mapping and candidate gene analysis of the floury endosperm gene, FLO(a), in rice.MOLECULES AND CELLS,29(2),167-174.
MLA	Qiao, Yongli,et al."Fine mapping and candidate gene analysis of the floury endosperm gene, FLO(a), in rice".MOLECULES AND CELLS 29.2(2010):167-174.