Isolation and Characterization of Maize PMP3 Genes Involved in Salt Stress Tolerance

doi:10.1371/journal.pone.0031101

	Isolation and Characterization of Maize PMP3 Genes Involved in Salt Stress Tolerance
	Fu, Jing 1,2; Zhang, Deng-Feng 2; Liu, Ying-Hui 2; Ying, Sheng 2; Shi, Yun-Su 2; Song, Yan-Chun 2; Li, Yu 2; Wang, Tian-Yu 2
刊名	PLOS ONE
	2012
卷号	7 期号:2 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0031101
通讯作者	Fu, Jing
英文摘要	Plasma membrane protein 3 (PMP3), a class of small hydrophobic polypeptides with high sequence similarity, is responsible for salt, drought, cold, and abscisic acid. These small hydrophobic ploypeptides play important roles in maintenance of ion homeostasis. In this study, eight ZmPMP3 genes were cloned from maize and responsive to salt, drought, cold and abscisic acid. The eight ZmPMP3s were membrane proteins and their sequences in trans-membrane regions were highly conserved. Phylogenetic analysis showed that they were categorized into three groups. All members of group II were responsive to ABA. Functional complementation showed that with the exception of ZmPMP3-6, all were capable of maintaining membrane potential, which in turn allows for regulation of intracellular ion homeostasis. This process was independent of the presence of Ca2+. Lastly, over-expression of ZmPMP3-1 enhanced growth of transgenic Arabidopsis under salt condition. Through expression analysis of deduced downstream genes in transgenic plants, expression levels of three ion transporter genes and four important antioxidant genes in ROS scavenging system were increased significantly in transgenic plants during salt stress. This tolerance was likely achieved through diminishing oxidative stress due to the possibility of ZmPMP3-1's involvement in regulation of ion homeostasis, and suggests that the modulation of these conserved small hydrophobic polypeptides could be an effective way to improve salt tolerance in plants.
学科主题	Multidisciplinary Sciences ; MULTIDISCIPLINARY SCIENCES
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000302733900022
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5200]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.China Agr Univ, Coll Biol Sci, Beijing 100094, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Fu, Jing,Zhang, Deng-Feng,Liu, Ying-Hui,et al. Isolation and Characterization of Maize PMP3 Genes Involved in Salt Stress Tolerance[J]. PLOS ONE,2012,7(2):-.
APA	Fu, Jing.,Zhang, Deng-Feng.,Liu, Ying-Hui.,Ying, Sheng.,Shi, Yun-Su.,...&Wang, Tian-Yu.(2012).Isolation and Characterization of Maize PMP3 Genes Involved in Salt Stress Tolerance.PLOS ONE,7(2),-.
MLA	Fu, Jing,et al."Isolation and Characterization of Maize PMP3 Genes Involved in Salt Stress Tolerance".PLOS ONE 7.2(2012):-.