Development of mapped simple sequence repeat markers from common bean (Phaseolus vulgaris L.) based on genome sequences of a Chinese landrace and diversity evaluation | |
Chen, Mingli1; Wu, Jing1; Wang, Lanfeng1; Zhang, Xiaoyan2; Blair, Matthew W.3; Jia, Jizeng1; Wang, Shumin1 | |
刊名 | MOLECULAR BREEDING |
2014 | |
卷号 | 33期号:2页码:489-496 |
关键词 | Phaseolus vulgaris L. SSR markers Genetic mapping Genome sequence |
ISSN号 | 1380-3743 |
DOI | 10.1007/s11032-013-9949-2 |
通讯作者 | Chen, Mingli |
英文摘要 | Microsatellite or single sequence repeat (SSR) markers have been commonly used in genetic research in many crop species, including common bean (Phaseolus vulgaris L.). A limited number of existing SSR markers have been designed from high-throughput sequencing of the genome, warranting the exploitation of new SSR markers from genomic regions. In this paper, we sequenced total DNA from the genotype Hong Yundou with a 454-FLX pyrosequencer and found numerous SSR loci. Based on these, a large number of SSR markers were developed and 90 genomic-SSR markers with clear bands were tested for mapping and diversity detection. The new SSR markers proved to be highly polymorphic for molecular polymorphism, with an average polymorphism information content value of 0.44 in 131 Chinese genotypes and breeding lines, effective for distinguishing Andean and Mesoamerican genotypes. In addition, we integrated 85 primers of the 90 polymorphism markers into the bean map using an F2 segregating population derived from Hong Yundou crossed with Jingdou. The distribution of SSR markers among 11 chromosomes was not random and tended to cluster on the linkage map, with 14 new markers mapped on chromosome Pv01, whereas only four loci were located on chromosome Pv04. Overall, these new markers have potential for genetic mapping, genetic diversity studies and map-based cloning in common bean. |
学科主题 | Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000330792100019 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/4931] |
专题 | 作物科学研究所_种质资源保存与研究中心 |
作者单位 | 1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Qingdao Acad Agr Sci, Qingdao 266100, Shandong, Peoples R China 3.Cornell Univ, Dept Plant Breeding, Ithaca, NY 14853 USA |
推荐引用方式 GB/T 7714 | Chen, Mingli,Wu, Jing,Wang, Lanfeng,et al. Development of mapped simple sequence repeat markers from common bean (Phaseolus vulgaris L.) based on genome sequences of a Chinese landrace and diversity evaluation[J]. MOLECULAR BREEDING,2014,33(2):489-496. |
APA | Chen, Mingli.,Wu, Jing.,Wang, Lanfeng.,Zhang, Xiaoyan.,Blair, Matthew W..,...&Wang, Shumin.(2014).Development of mapped simple sequence repeat markers from common bean (Phaseolus vulgaris L.) based on genome sequences of a Chinese landrace and diversity evaluation.MOLECULAR BREEDING,33(2),489-496. |
MLA | Chen, Mingli,et al."Development of mapped simple sequence repeat markers from common bean (Phaseolus vulgaris L.) based on genome sequences of a Chinese landrace and diversity evaluation".MOLECULAR BREEDING 33.2(2014):489-496. |
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