Development and validation of molecular markers closely linked to the wheat stripe rust resistance gene YrC591 for marker-assisted selection | |
Xu, Hongxing1; Zhang, Jie1; Zhang, Ping1; Qie, Yanmin1; Niu, Yongchun2; Li, Hongjie3; Ma, Pengtao1; Xu, Yunfeng1; An, Diaoguo1 | |
刊名 | EUPHYTICA |
2014 | |
卷号 | 198期号:3页码:317-323 |
关键词 | Stripe rust resistance Puccinia striiformis SSR marker STS marker MAS Triticum aestivum |
ISSN号 | 0014-2336 |
DOI | 10.1007/s10681-014-1108-2 |
通讯作者 | Xu, Hongxing |
英文摘要 | Stripe rust resistance gene YrC591, present in wheat cultivar C591, is effective against currently important Puccinia striiformis Westend. f. sp. tritici isolates in China. An F-2:3 population (127 lines) was developed by crossing C591 with susceptible cultivar Taichung 29. Thirty four simple sequence repeat (SSR) and 155 sequence tagged site (STS) markers located on chromosome 7BL were used to perform bulk segregant analysis. Eight SSR markers, cfa2040, wmc273, wmc166, gwm984, barc32 wmc276, barc182 and gwm146, and 6 STS markers, mag1714, mag1757, mag1811, BE425120, BE471173 and BG607810, were polymorphic between the parents and contrasting resistant and susceptible DNA pools. F-2:3 lines were genotyped with these polymorphic markers. Linkage analysis indicated that YrC591 was flanked by Xmag1714 and Xbarc182 with genetic distances of 1.2 and 0.4 cM, respectively. In addition, validation of the SSR markers cfa2040, wmc273 and barc32, and STS markers mag1714 and BE425120 was carried out using wheat lines with C591 as a parent, indicating that these markers should be effective in tracing this gene in marker-assisted selection. |
学科主题 | Agronomy ; Plant Sciences ; Horticulture ; AGRONOMY ; HORTICULTURE ; PLANT SCIENCES |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000339804800001 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/4855] |
专题 | 作物科学研究所_种质资源保存与研究中心 |
作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Agr Resources Res, Key Lab Agr Water Resources, Shijiazhuang 050021, Peoples R China 2.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China |
推荐引用方式 GB/T 7714 | Xu, Hongxing,Zhang, Jie,Zhang, Ping,et al. Development and validation of molecular markers closely linked to the wheat stripe rust resistance gene YrC591 for marker-assisted selection[J]. EUPHYTICA,2014,198(3):317-323. |
APA | Xu, Hongxing.,Zhang, Jie.,Zhang, Ping.,Qie, Yanmin.,Niu, Yongchun.,...&An, Diaoguo.(2014).Development and validation of molecular markers closely linked to the wheat stripe rust resistance gene YrC591 for marker-assisted selection.EUPHYTICA,198(3),317-323. |
MLA | Xu, Hongxing,et al."Development and validation of molecular markers closely linked to the wheat stripe rust resistance gene YrC591 for marker-assisted selection".EUPHYTICA 198.3(2014):317-323. |
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