Transcriptomic Analysis of Drought Stress Responses in Ammopiptanthus mongolicus Leaves Using the RNA-Seq Technique

doi:10.1371/journal.pone.0124382

	Transcriptomic Analysis of Drought Stress Responses in Ammopiptanthus mongolicus Leaves Using the RNA-Seq Technique
	Gao, Fei 1; Wang, Jianyue 2; Wei, Shanjun 1; Li, Zhanglei 1; Wang, Ning 1; Li, Huayun 1; Feng, Jinchao 1; Li, Hongjie 3; Zhou, Yijun 1; Zhang, Feixiong 2
刊名	PLOS ONE
	2015
卷号	10 期号:4 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0124382
通讯作者	Gao, Fei
英文摘要	Ammopiptanthus mongolicus (Maxim. Ex Kom.) Cheng f., a relic tree of the Tertiary period, plays a critical role in maintaining desert ecosystems in the Mid-Asia region. Genome-scale gene expression profiling studies will provide deep insight into the molecular mechanism underlying the drought tolerance of A. mongolicus. In the present study, we investigated the transcriptional changes induced by drought treatment in A. mongolicus leaves by establishing a comprehensive transcriptome database and then performing a Digital Gene Expression (DGE) analysis using Solexa sequencing technology. A comprehensive transcriptome database was obtained by assembling the Illumina unigenes with expressed sequence tags (EST) available publicly, and other high throughput sequencing data. To analyze the dynamic and complicated gene regulation network during PEG6000-induced drought treatment in leaves of A. mongolicus, a time-course gene expression analysis was performed using tag-based DGE technology, which identified 437, 1,247 and 802 differentially expressed transcripts in 1, 24 and 72 h drought stress libraries, respectively. GO and KEGG analyses revealed hormone signal transduction and phenylpropanoid biosynthesis were enriched during drought treatment. A batch of drought-regulated transcription factor transcripts were identified, including the subsets of HD-ZIP, bZIP, WRKY, AP2/ERF and bHLH family members, which may play roles in drought response in A. mongolicus. The sequence collection assembled in the present study represents one of the most comprehensive transcriptome databases for A. mongolicus currently. The differentially expressed transcripts identified in our study provide a good start for identifying the key genes in stress response and performing functional analysis to reveal their roles in stress adaptation in planta.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000353711600080
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4726]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China 2.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Gao, Fei,Wang, Jianyue,Wei, Shanjun,et al. Transcriptomic Analysis of Drought Stress Responses in Ammopiptanthus mongolicus Leaves Using the RNA-Seq Technique[J]. PLOS ONE,2015,10(4):-.
APA	Gao, Fei.,Wang, Jianyue.,Wei, Shanjun.,Li, Zhanglei.,Wang, Ning.,...&Zhang, Feixiong.(2015).Transcriptomic Analysis of Drought Stress Responses in Ammopiptanthus mongolicus Leaves Using the RNA-Seq Technique.PLOS ONE,10(4),-.
MLA	Gao, Fei,et al."Transcriptomic Analysis of Drought Stress Responses in Ammopiptanthus mongolicus Leaves Using the RNA-Seq Technique".PLOS ONE 10.4(2015):-.