CRYOPRESERVATION OF IN VITRO-GROWN SHOOT TIPS OF CHINESE MEDICINAL PLANT Atractylodes macrocephala KOIDZ. USING A DROPLET-VITRIFICATION METHOD

	CRYOPRESERVATION OF IN VITRO-GROWN SHOOT TIPS OF CHINESE MEDICINAL PLANT Atractylodes macrocephala KOIDZ. USING A DROPLET-VITRIFICATION METHOD
	Zhang, Jin-mei 1; Huang, Bin 2; Lu, Xin-xiong 1; Volk, Gayle M.3; Xin, Xia 1; Yin, Guang-kun 1; He, Juan-juan 1; Chen, Xiao-ling 1
刊名	CRYOLETTERS
	2015
卷号	36 期号:3 页码:195-204
关键词	Atractylodes macrocephala Koidz. droplet-vitrification regrowth shoot tips survival thermal analysis
ISSN号	0143-2044
通讯作者	Zhang, Jin-mei
英文摘要	BACKGROUND: Atractylodes macrocephala Koidz. is an important medicinal species from China that has been used for thousands of years for its special pharmacological antioxidant, hepatoprotective, anti-inflammatory, anti-allergic, antithrombotic, antiviral, and anticarcinogenic activities. OBJECTIVE: The aim of this research was to develop an efficient droplet-vitrification protocol for A. macrocephala shoot tips which could be used as a strategy for long-term conservation within gene banks. MATERIALS AND METHODS: The duration of preculture, loading, and PVS2 steps, as well as the recovery medium formulation, were optimized to achieve high levels of survival and regrowth for A. macrocephala shoot tips after liquid nitrogen exposure. RESULTS: Survival and regrowth levels after cryopreservation in the cultivar 'Baizhu' were as high as 76% and 62%, respectively. Thermal analysis using differential scanning calorimetry suggested that the PVS2 treatment plays a critical role for successful cryopreservation. CONCLUSION: The droplet-vitrification method established in this study could be used to cryopreserve A. macrocephala.
学科主题	Biology ; Physiology
语种	英语
出版者	CRYO LETTERS
WOS记录号	WOS:000357552300007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4719]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Genebank, Beijing 100081, Peoples R China 2.Fujian Agr & Forestry Univ, Fuzhou 350002, Peoples R China 3.USDA ARS, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA
推荐引用方式 GB/T 7714	Zhang, Jin-mei,Huang, Bin,Lu, Xin-xiong,et al. CRYOPRESERVATION OF IN VITRO-GROWN SHOOT TIPS OF CHINESE MEDICINAL PLANT Atractylodes macrocephala KOIDZ. USING A DROPLET-VITRIFICATION METHOD[J]. CRYOLETTERS,2015,36(3):195-204.
APA	Zhang, Jin-mei.,Huang, Bin.,Lu, Xin-xiong.,Volk, Gayle M..,Xin, Xia.,...&Chen, Xiao-ling.(2015).CRYOPRESERVATION OF IN VITRO-GROWN SHOOT TIPS OF CHINESE MEDICINAL PLANT Atractylodes macrocephala KOIDZ. USING A DROPLET-VITRIFICATION METHOD.CRYOLETTERS,36(3),195-204.
MLA	Zhang, Jin-mei,et al."CRYOPRESERVATION OF IN VITRO-GROWN SHOOT TIPS OF CHINESE MEDICINAL PLANT Atractylodes macrocephala KOIDZ. USING A DROPLET-VITRIFICATION METHOD".CRYOLETTERS 36.3(2015):195-204.