Structural features and immunostimulating effects of three acidic polysaccharides isolated from Panax quinquefolius

doi:10.1016/j.ijbiomac.2015.06.007

	Structural features and immunostimulating effects of three acidic polysaccharides isolated from Panax quinquefolius
	Wang, Lijun 1,2; Yao, Yang 1; Sang, Wei 1; Yang, Xiushi 1; Ren, Guixing 1
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2015
卷号	80 页码:77-86
关键词	American ginseng Acidic polysaccharides C-13 NMR Immuno-modulatory activities Macrophages
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2015.06.007
通讯作者	Wang, Lijun
英文摘要	Three acidic polysaccharides (PPQA2, PPQA4 and PPQA5) were successfully purified from the water-extracted crude polysaccharides of Panax quinquefolius by combination of DEAE Sepharose and Sephacryl S-300 chromatography. The average molecular weights (Mws) of PPQA2, PPQA4 and PPQA5 were 2.3 x 10(4) Da, 1.2 x 10(5) Da, and 5.3 x 10(3) Da, respectively. Monosaccharides components analysis indicated that PPQA2 and PPQA5 were composed of arabinose, rhammose, mannose, galactose, glucose, galacturonic acid and glucuronic acid in a molar percent of 8.0:4.0:2.9:7.2:12.5:26.6:38.8 and 8.5:3.2:5.3:10.8:32.4:15.5:24.4. PPQA4 was composed of arabinose, rhammose, mannose, galactose, glucose and glucuronic acid in a molar percent of 19.7:5.1:8.1:23.9:41.3:2.0. The C-13 NMR spectra indicated the existence of O-acetyl groups in PPQA2 and PPQA5, while absence in PPQA4. The in vitro study showed that PPQA2, PPQA4 and PPQA5 were all able to stimulate the production of secretory molecules (NO, TNF-alpha, and IL-6) of RAW264.7 murine macrophages in a dose-dependent manner. These findings suggest that the three acidic polysaccharides isolated in our study have immunopotentiating effects on macrophages and should be used as a beneficial health food. (C) 2015 Elsevier E.V. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000361251000010
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4673]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 2.Chinese Univ Hong Kong, Sch Life Sci, Food & Nutr Sci Programme, Shatin, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Lijun,Yao, Yang,Sang, Wei,et al. Structural features and immunostimulating effects of three acidic polysaccharides isolated from Panax quinquefolius[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2015,80:77-86.
APA	Wang, Lijun,Yao, Yang,Sang, Wei,Yang, Xiushi,&Ren, Guixing.(2015).Structural features and immunostimulating effects of three acidic polysaccharides isolated from Panax quinquefolius.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,80,77-86.
MLA	Wang, Lijun,et al."Structural features and immunostimulating effects of three acidic polysaccharides isolated from Panax quinquefolius".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 80(2015):77-86.