Effect of ultrasonic treatment on immunological activities of polysaccharides from adlay

doi:10.1016/j.ijbiomac.2015.06.033

	Effect of ultrasonic treatment on immunological activities of polysaccharides from adlay
	Yao, Yang 1; Zhu, Yingying 1,2; Gao, Yue 1,3; Ren, Guixing 1
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2015
卷号	80 页码:246-252
关键词	Adlay Alkali-extractable polysaccharide Ultrasonic
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2015.06.033
通讯作者	Yao, Yang
英文摘要	Alkali-extractable polysaccharides from the seed of adlay and their two polysaccharide sub-fractions (AAP-1 and AAP-2) were isolated and purified by anion-exchange and gel filtration chromatography. Ultrasonic treatment was put in place to obtain AAP-1' and AAP-2'. Average molecular weights (Mws) of AAP-1, AAP-1', AAP-2, and AAP-2' were 94.2 kDa, 63.1 kDa, 82.3 kDa, and 60.4 kDa, respectively. Monosaccharides composition analysis indicated that AAP-1 and AAP-1' were composed of Rha, Ara, Glc, and Gal in a molar ratio of 1.1:0.4:0.7:0.5 and 1.4:1.6:0.4:0.7. AAP-2 and AAP-2' were composed of Xyl, Rha, Gal and Glc in a molar percent of 0.4:1.4:1.6:0.5 and 0.3:1.6:1.1:0.7. In vitro study showed that AAP-1, AAP-1', AAP-2, and AAP-2' were all able to encourage the production of secretory molecules (NO, TNF-alpha and IL-6) of RAW264.7 murine macrophages in concentrations determines manner. AAP-2' seemed to be the most potent and induced significantly higher the NO production. These findings suggest that the ultrasonic polysaccharides isolated in our study have immune potentiation effects on macrophages and should serve as a beneficial health food. (C) 2015 Elsevier B.V. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000361251000028
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4672]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 2.Univ Liege, Lab Biophys & Formulat Engn, Gembloux Agrobio Tech, B-5030 Gembloux, Belgium 3.Qilu Univ Technol, Jinan 250353, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Yao, Yang,Zhu, Yingying,Gao, Yue,et al. Effect of ultrasonic treatment on immunological activities of polysaccharides from adlay[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2015,80:246-252.
APA	Yao, Yang,Zhu, Yingying,Gao, Yue,&Ren, Guixing.(2015).Effect of ultrasonic treatment on immunological activities of polysaccharides from adlay.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,80,246-252.
MLA	Yao, Yang,et al."Effect of ultrasonic treatment on immunological activities of polysaccharides from adlay".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 80(2015):246-252.