Suppressive Effects of Barley beta-Glucans with Different Molecular Weight on 3T3-L1 Adipocyte Differentiation

doi:10.1111/1750-3841.13226

	Suppressive Effects of Barley beta-Glucans with Different Molecular Weight on 3T3-L1 Adipocyte Differentiation
	Zhu, Yingying 1,2; Yao, Yang 1; Gao, Yue 1,3; Hu, Yibo 1; Shi, Zhenxing 1; Ren, Guixing 1
刊名	JOURNAL OF FOOD SCIENCE
	2016
卷号	81 期号:3 页码:H786-H793
关键词	barley beta-glucans 3T3-L1 cells PPAR gamma C/EBP alpha
ISSN号	0022-1147
DOI	10.1111/1750-3841.13226
通讯作者	Zhu, Yingying
英文摘要	In this study, 2 beta-glucans with different molecular weight were prepared and purified from hull-less barley bran. The aim was to evaluate their effects on the differentiation of 3T3-L1 pre-adipocytes. Results showed that barley beta-glucans inhibited the differentiation of 3T3-L1 pre-adipocytes induced by differentiation medium in a dose-dependent manner, the suppressive effect of high-molecular-weight barley beta-glucans (552 kDa, BGH) was stronger (P < 0.05) than that of low-molecular-weight barley beta-glucan (32 kDa, BGL), evidenced by the significantly decrease (P < 0.05) of Oil-red O staining and intracellular triglyceride content in the mature adipocytes. Besides, gene expression analysis and Western Blot analysis revealed that both BGH and BGL inhibited the mRNA and protein levels of adipogenesis related transcription factors peroxisome proliferator-activated receptor. (PPAR.) and CCAAT-enhancer-binding protein a (C/EBP alpha) which are principal regulators of adipogenesis. Furthermore, the mRNA and protein expression levels of PPAR. target genes in adipose tissue including adipocyte fatty acid binding protein (ap2), lipoprotein lipase (LPL), uncoupling protein-2 (UCP-2), and glucose-transporter 4 (Glut4) in 3T3-L1 cells was also markedly downregulated (P < 0.05). These findings were anticipated to help develop barley beta-glucans based functional food for the management of obesity.
学科主题	Food Science & Technology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000372148400018
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4569]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 2.Univ Liege, Gembloux Agrobio Tech, Anim Sci Unit, Passage Deportes 2, B-5030 Gembloux, Belgium 3.Western Univ, Qilu Univ Technol, Daxue Rd,Sci Pk, Jinan 250353, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Yingying,Yao, Yang,Gao, Yue,et al. Suppressive Effects of Barley beta-Glucans with Different Molecular Weight on 3T3-L1 Adipocyte Differentiation[J]. JOURNAL OF FOOD SCIENCE,2016,81(3):H786-H793.
APA	Zhu, Yingying,Yao, Yang,Gao, Yue,Hu, Yibo,Shi, Zhenxing,&Ren, Guixing.(2016).Suppressive Effects of Barley beta-Glucans with Different Molecular Weight on 3T3-L1 Adipocyte Differentiation.JOURNAL OF FOOD SCIENCE,81(3),H786-H793.
MLA	Zhu, Yingying,et al."Suppressive Effects of Barley beta-Glucans with Different Molecular Weight on 3T3-L1 Adipocyte Differentiation".JOURNAL OF FOOD SCIENCE 81.3(2016):H786-H793.