Rapid determination of fatty acids in soybeans [Glycine max (L.) Merr.] by FT-near-infrared reflectance spectroscopy

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	Rapid determination of fatty acids in soybeans [Glycine max (L.) Merr.] by FT-near-infrared reflectance spectroscopy
	Sun Jun-ming 1; Han Fen-xia 1; Yan Shu-rong 1; Yang Hua 1; Tetsuo Sato 2
刊名	SPECTROSCOPY AND SPECTRAL ANALYSIS
	2008
卷号	28 期号:6 页码:1290-1295
关键词	soybean [Glycine max (L.) Merr.] fatty acid FT-NIRS gas chromatograph
ISSN号	1000-0593
通讯作者	Sun Jun-ming
英文摘要	Current breeding programs dealing with fatty acid (FA) concentrations in soybean [Glycine max (L.) Merr.] require large numbers for gas chromatographic analyses, thus it is important to develop a method for rapid determination of fatty acid by Near-Infrared Reflectance spectroscopy (NIRS) in soybeans. The objective of this work was to study the potential of fourier-transform near-infrared reflectance spectroscopy (FT-NIRS) to estimate the fatty acid concentrations in Chinese soybean varieties. One hundred and eight of soybean cultivars or lines (the calibration set: 64; the external validation set: 44) were scanned within 4 000-12 500 cm(-1) of wavenumbers using a standard sample cup by NIRS machinery, and analyzed the fatty acids by gas chromatograph (GC) methods. Equations were developed using partial least squares (PLS) regression and cross validation for multivariate calibration in this study. The optimal spectral region was selected from 6 101.9 to 5 446.5 cm(-1) based on the OPUS 5.0 software. Cross validation results showed that major FA components such as oleic acid (R-cv(2) = 0.94), linoleic acid (R-cv(2) = 0.87), linolenic acid (R-cv(2) = 0.85), and total saturates (R-cv(2) = 0.88) were accurately determined by the proposed equations as compared with the reference data obtained by the GC method. External validation results also demonstrated that equation for oleic acid had the highest predictive ability (R-val(2) = 0.91), root mean square error of predication (RMSEP) value was 2.47 g (.) kg(-1) dry weight, the ratios of RMSEP to the standard deviation (SD) was 0.29, which was usable for quality assurance application. Moreover, equations for palmitic acid, stearic acid, linoleic acid, linolenic acid, and total saturates were predicted with the determination coefficients ranging from 0.66 to 0.76, RMSEP values from 0.37 to 2.74 g (.) kg(-1) dry weight, and RMSEP/SD values from 0.47 to 0.53, which could be used for sample screening. Therefore, we confirmed that a reliable estimation of the major fatty acid components is possible by using NIRS technique in soybeans.
学科主题	Spectroscopy ; SPECTROSCOPY
语种	中文
出版者	BEIJING UNIV PRESS
WOS记录号	WOS:000257356600021
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5602]
专题	作物科学研究所_职能部门
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Natl Agr Res Ctr Kyushu Okinawa Reg KONARC, Koshi City, Kumamoto 8611192, Japan
推荐引用方式 GB/T 7714	Sun Jun-ming,Han Fen-xia,Yan Shu-rong,et al. Rapid determination of fatty acids in soybeans [Glycine max (L.) Merr.] by FT-near-infrared reflectance spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2008,28(6):1290-1295.
APA	Sun Jun-ming,Han Fen-xia,Yan Shu-rong,Yang Hua,&Tetsuo Sato.(2008).Rapid determination of fatty acids in soybeans [Glycine max (L.) Merr.] by FT-near-infrared reflectance spectroscopy.SPECTROSCOPY AND SPECTRAL ANALYSIS,28(6),1290-1295.
MLA	Sun Jun-ming,et al."Rapid determination of fatty acids in soybeans [Glycine max (L.) Merr.] by FT-near-infrared reflectance spectroscopy".SPECTROSCOPY AND SPECTRAL ANALYSIS 28.6(2008):1290-1295.