SPECIFIC AND SENSITIVE DETECTION OF THE FUNGAL PATHOGEN USTILAGINOIDEA VIRENS BY NESTED PCR

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	SPECIFIC AND SENSITIVE DETECTION OF THE FUNGAL PATHOGEN USTILAGINOIDEA VIRENS BY NESTED PCR
	Zhou Yongli
刊名	菌物学报
	2004
卷号	23 期号:1 页码:102-108
关键词	Rice false smut ITS PCR primers
ISSN号	1672-6472
其他题名	稻曲病菌的Nested PCR检测
英文摘要	A 624-625 bp fragment of the inner space region of ITS and 5.8S rDNA of strains of Ustilaginoidea virens were sequenced, which collected from diverse rice varieties and geographical origins in different years in order to design PCR primers for its detection. Alignment of the sequence data of all collections revealed the ITS region of this pathogen was almost completely conservative. Based on the comparison with the ITS sequences of other Clavicipitaceous fungi, specific primers for U. virens were developed. The objective fungus was successfully detected with its specific primer set with high specificity and sensitivity, and the PCR results correlated well with assessments based on conventional technique of histological inspection. This result could provide a useful technique to study disease cycle and early prediction of field incidence of false smut.
学科主题	微生物学
语种	英语
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/106382]
专题	作物科学研究所
作者单位	中国农业科学院作物育种栽培所, Key Laboratory of Crop Genetic and Breeding of Ministry of Agriculture, CAAS, 北京, 100081
推荐引用方式 GB/T 7714	Zhou Yongli. SPECIFIC AND SENSITIVE DETECTION OF THE FUNGAL PATHOGEN USTILAGINOIDEA VIRENS BY NESTED PCR[J]. 菌物学报,2004,23(1):102-108.
APA	Zhou Yongli.(2004).SPECIFIC AND SENSITIVE DETECTION OF THE FUNGAL PATHOGEN USTILAGINOIDEA VIRENS BY NESTED PCR.菌物学报,23(1),102-108.
MLA	Zhou Yongli."SPECIFIC AND SENSITIVE DETECTION OF THE FUNGAL PATHOGEN USTILAGINOIDEA VIRENS BY NESTED PCR".菌物学报 23.1(2004):102-108.