Identification and functional characterization of the AGO1 ortholog in maize

doi:10.1111/jipb.12467

CORC > 中国农业科学院 > 作物科学研究所

	Identification and functional characterization of the AGO1 ortholog in maize
	Xu Dongdong; Yang Hailong; Zou Cheng; Li Wenxue; Xu Yunbi; Xie Chuanxiao
刊名	Journal of Integrative Plant Biology
	2016
卷号	58 期号:8 页码:749-758
关键词	AGO1 functional complementation maize ZmAGO1a
ISSN号	1672-9072
DOI	10.1111/jipb.12467
英文摘要	Eukaryotic Argonaute proteins play primary roles in miRNA and siRNA pathways that are essential for numerous developmental and biological processes. However, the functional roles of the four ZmAGO1 genes have not yet been characterized in maize (Zea mays L.). In the present study, ZmAGO1a was identified from four putative ZmAGO1 genes for further characterization. Complementation of the Arabidopsis ago1-27 mutant with ZmAGO1a indicated that constitutive overexpression of ZmAGO1a could restore the smaller rosette, serrated leaves, later flowering and maturation, lower seed set, and darker green leaves at late stages of the mutant to the wild-type phenotype. The expression profiles of ZmAGO1a under five different abiotic stresses indicated that ZmAGO1a shares expression patterns similar to those of Argonaute genes in rice, Arabidopsis, and wheat. Further, variation in ZmAGO1a alleles among diverse maize germplasm that resulted in several amino acid changes revealed genetic diversity at this locus. The present data suggest that ZmAGO1a might be an important AGO1 ortholog in maize. The results presented provide further insight into the function of ZmAGO1a.
学科主题	植物学
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101665]
专题	作物科学研究所
作者单位	Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081
推荐引用方式 GB/T 7714	Xu Dongdong,Yang Hailong,Zou Cheng,et al. Identification and functional characterization of the AGO1 ortholog in maize[J]. Journal of Integrative Plant Biology,2016,58(8):749-758.
APA	Xu Dongdong,Yang Hailong,Zou Cheng,Li Wenxue,Xu Yunbi,&Xie Chuanxiao.(2016).Identification and functional characterization of the AGO1 ortholog in maize.Journal of Integrative Plant Biology,58(8),749-758.
MLA	Xu Dongdong,et al."Identification and functional characterization of the AGO1 ortholog in maize".Journal of Integrative Plant Biology 58.8(2016):749-758.