Analysis of the genetic structure of Sclerotinia sclerotiorum (Lib.) de Bary - Populations from different regions and host plants by random amplified polymorphic DNA markers

doi:10.1111/j.1744-7909.2005.00077.x

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	Analysis of the genetic structure of Sclerotinia sclerotiorum (Lib.) de Bary - Populations from different regions and host plants by random amplified polymorphic DNA markers
	Sun, JM; Irzykowski, W; Jedryczka, M; Han, FX
刊名	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
	2005
卷号	47 期号:4 页码:385-395
关键词	genetic structure intraspecific variability Sclerotinia sclerotiorum (Lib.) de Bary random amplified polymorphic DNA (RAPD) analysis
ISSN号	1672-9072
DOI	10.1111/j.1744-7909.2005.00077.x
通讯作者	Sun, JM
英文摘要	The genetic diversity and genetic structure of a population of isolates of Sclerotinia sclerotiorum (Lib.) de Bary from different regions and host plants were investigated using the random amplified polymorphic DNA (RAPD) method with 20 random decamer primer pairs in order to provide some information on the phylogenetic taxa and breeding for resistance to sclerotinia stem rot. A minimum of three and a maximum of 15 unambiguously amplified bands were generated, furnishing a total of 170 bands ranging in size from 100 to 3 200 bp, corresponding to an average of 8.5 bands per primer pair. One hundred and four of these 170 bands (61.2%) were polymorphic, the percentage of polymorphic bands for each primer pair ranging from 0.0% to 86.7%. The genetic relationships among the isolates, based on the results of RAPD analysis, were examined. The genetic similarity of all selected isolates was quite high. At the species level, the genetic diversity estimated by Nei ' s gene diversity (h) was 0.197 and Shannon ' s index of diversity (I) was 0.300. The unweighted pair-group mean analysis (UPGMA) cluster analysis showed that most isolates from the same regions were grouped in the same cluster or a close cluster. The population of isolates from Hefei (Anhui Province, China) was more uniform and relatively distant to other populations. The Canadian population collected from carrot (Daucus carota var. sativa DC.) was relatively close to the Polish population collected from oilseed rape (Brassica napus L.) plants. There was no relationship between isolates from the same host plants. An analysis of molecular variance (AMOVA) revealed that the percentage of variance attributable to variation among and within populations was 50.62% and 49.38%, respectively. When accessions from China, Europe, and Canada were treated as three separate groups, the variance components among groups, among populations within groups, and within populations were -0.96%, 51.48%, and 49.47%, respectively. The genetic differentiations among and within populations were highly significant (P < 0.00 1). Similarly, the coefficient of gene differentiation (Gst) in total populations calculated by population genetic analysis was 0.229 4, which indicated that the genetic variation among populations was 22.94%. The gene flow (Nm) was 1.68, which indicated that the gene permutation and interaction among populations was relatively high.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; PLANT SCIENCES
语种	英语
出版者	BLACKWELL PUBLISHING ASIA
WOS记录号	WOS:000228185500001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5776]
专题	作物科学研究所
作者单位	(1)Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Crop Genet & Breeding, Minist Agr, Beijing 100081, Peoples R China;(2)Polish Acad Sci, Inst Plant Genet, PL-60479 Poznan, Poland
推荐引用方式 GB/T 7714	Sun, JM,Irzykowski, W,Jedryczka, M,et al. Analysis of the genetic structure of Sclerotinia sclerotiorum (Lib.) de Bary - Populations from different regions and host plants by random amplified polymorphic DNA markers[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2005,47(4):385-395.
APA	Sun, JM,Irzykowski, W,Jedryczka, M,&Han, FX.(2005).Analysis of the genetic structure of Sclerotinia sclerotiorum (Lib.) de Bary - Populations from different regions and host plants by random amplified polymorphic DNA markers.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,47(4),385-395.
MLA	Sun, JM,et al."Analysis of the genetic structure of Sclerotinia sclerotiorum (Lib.) de Bary - Populations from different regions and host plants by random amplified polymorphic DNA markers".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 47.4(2005):385-395.