A novel STS marker for polyphenol oxidase activity in bread wheat

doi:10.1007/s11032-005-6618-0

CORC > 中国农业科学院 > 作物科学研究所

	A novel STS marker for polyphenol oxidase activity in bread wheat
	Sun, DJ; He, ZH; Xia, XC; Zhang, LP; Morris, CF; Appels, R; Ma, WJ; Wang, H
刊名	MOLECULAR BREEDING
	2005
卷号	16 期号:3 页码:209-218
关键词	bread wheat marker-assisted selection polyphenol oxidase activity STS marker
ISSN号	1380-3743
DOI	10.1007/s11032-005-6618-0
通讯作者	Sun, DJ
英文摘要	The enzyme activity of polyphenol oxidase (PPO) in grain has been related to undersirable brown discoloration of bread wheat (Triticum aestivum L.) based end-products, particularly for Asian noodles. Breeding wheat cultivars with low PPO activity is the best approach to reduce the undesirable darkening. Molecular markers could greatly improve selection efficiency in breeding programs. Based on the sequences of PPO genes (GenBank Accession Numbers AY596268, AY596269 and AY596270) conditioning PPO activity during kernel development, 28 pairs of primers were designed using the software 'DNAMAN'. One of the markers from AY596268, designated as PPO18, can amplify a 685-bp and an 876-bp fragment in the cultivars with high and low PPO activity, respectively. The difference of 191-bp size was detected in the intron region of the PPO gene. The STS marker PPO18 was mapped to chromosome 2AL using a DH population derived from a cross Zhongyou 9507 x CA9632, a set of nulli-tetrasomic lines and ditelosomic line 2AS of Chinese Spring. QTL analysis indicated that the PPO gene co-segregated with the STS marker PPO18 and is closely linked to Xgwm312 and Xgwm294 on chromosome 2AL, explaining 28-43% of phenotypic variance for PPO activity across three environments. A total of 233 Chinese wheat cultivars and advanced lines were used to validate the correlation between the polymorphic fragments of PPO18 and grain PPO activity. The results showed that PPO18 is a co-dominant, efficient and reliable molecular marker for PPO activity and can be used in wheat breeding programs targeted for noodle quality improvement.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000233531900003
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5763]
专题	作物科学研究所
作者单位	(1)Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Beijing 100081, Peoples R China;(2)Washington State Univ, USDA ARS, Western Wheat Qual Lab, Pullman, WA 99164 USA;(3)Murdoch Univ, Western Australia Dept Agr, Mol Plant Breeding CRC, Perth, WA 6150, Australia;(4)NW Sci Tech Univ Agr & Forestry, Coll Agron, Yangling, Peoples R China;(5)Chinese Acad Agr Sci, CIMMYT China Off, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Sun, DJ,He, ZH,Xia, XC,et al. A novel STS marker for polyphenol oxidase activity in bread wheat[J]. MOLECULAR BREEDING,2005,16(3):209-218.
APA	Sun, DJ.,He, ZH.,Xia, XC.,Zhang, LP.,Morris, CF.,...&Wang, H.(2005).A novel STS marker for polyphenol oxidase activity in bread wheat.MOLECULAR BREEDING,16(3),209-218.
MLA	Sun, DJ,et al."A novel STS marker for polyphenol oxidase activity in bread wheat".MOLECULAR BREEDING 16.3(2005):209-218.