Characterization of three low-molecular-weight Glu-D3 subunit genes in common wheat

doi:10.1007/s00122-006-0379-y

CORC > 中国农业科学院 > 作物科学研究所

	Characterization of three low-molecular-weight Glu-D3 subunit genes in common wheat
	Zhao, X. L.; Xia, X. C.; He, Z. H.; Gale, K. R.; Lei, Z. S.; Appels, R.; Ma, W.
刊名	THEORETICAL AND APPLIED GENETICS
	2006
卷号	113 期号:7 页码:1247-1259
ISSN号	0040-5752
DOI	10.1007/s00122-006-0379-y
通讯作者	Zhao, X. L.
英文摘要	Low-molecular-weight glutenins (LMW-GS) in common wheat (Triticum aestivum L.) are of great importance for processing quality of pan bread and noodles. The objectives of this study are to identify LMW-GS coding genes at GluD3 locus on chromosome 1D and to establish relationships between these genes and GluD3 alleles (a, b, c, d, and e) defined by protein electrophoretic mobility. Specific primer sets were designed to amplify each of the three LMW-GS chromosome 1D gene regions including upstream, coding and downstream regions of eight wheat cultivars containing GluD3 a, b, c, d and e alleles. Three LMW-GS genes, designated as GluD3-1, GluD3-2 and GluD3-3, were amplified from the eight wheat cultivars. The allelic variants of these three genes were analysed at the DNA and protein level. GluD3-1 showed two allelic variants or haplotypes, one common to cultivars containing protein alleles a, d and e (designated GluD3-11) and the other was present in cultivars with alleles b and c (designated GluD3-12). Comparing with GluD3-12, a 3-bp deletion was found in the coding region of the N-terminal repetitive domain of GluD3-11, leading to a glutamine deletion at the 116th position. GluD3-2 had three variants at the DNA level in the eight cultivars, which were designated as GluD3-21, GluD3-22 and GluD3-23. In comparison to GluD3-21, a single nucleotide polymorphism (SNP) was detected for GluD3-22 in the signal peptide region, resulting in an amino acid change from alanine to threonine at the 11th position; and 11 mutations were found at GluD3-23, with five in upstream region, four in coding region and two in downstream region, respectively. GluD3-3 had two haplotypes, designated as GluD3-31 and GluD3-32, both belonging to LMW-s glutenin subunits though their first amino acids in N-terminal region are different. Compared with the GenBank GluD3 genes, nucleotide sequences of GluD3-21 and GluD3-23 were the same as X13306 and AB062875, respectively. GluD3-22 and GluD3-11 had only one-base difference from U86027 and AB062865. GluD3-12 was not found in the GenBank database, indicating a newly identified GluD3 gene variation. GluD3-3 was a new gene different from any other known GluD3 genes. Analyses of the relationship between Glu-D3 alleles defined by protein electrophoretic mobility and different GluD3 gene variations at the DNA or protein level provided molecular basis for DNA based identification of glutenin alleles.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000241261000007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5713]
专题	作物科学研究所
作者单位	(1)Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Beijing 100081, Peoples R China;(2)Murdoch Univ, Western Australia Dept Agr & Mol Plant Breeding C, State Agr Biotechnol Ctr, Murdoch, WA 1650, Australia;(3)Henan Acad Agr Sci, Wheat Res Inst, Zhengzhou 450002, Henan, Peoples R China;(4)CSIRO Plant Ind, Canberra, ACT 2601, Australia;(5)Chinese Acad Agr Sci, Int Maize & Wheat Improvement Ctt, CIMMYT, China Off, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhao, X. L.,Xia, X. C.,He, Z. H.,et al. Characterization of three low-molecular-weight Glu-D3 subunit genes in common wheat[J]. THEORETICAL AND APPLIED GENETICS,2006,113(7):1247-1259.
APA	Zhao, X. L..,Xia, X. C..,He, Z. H..,Gale, K. R..,Lei, Z. S..,...&Ma, W..(2006).Characterization of three low-molecular-weight Glu-D3 subunit genes in common wheat.THEORETICAL AND APPLIED GENETICS,113(7),1247-1259.
MLA	Zhao, X. L.,et al."Characterization of three low-molecular-weight Glu-D3 subunit genes in common wheat".THEORETICAL AND APPLIED GENETICS 113.7(2006):1247-1259.