Overexpression of a putative maize calcineurin B-like protein in Arabidopsis confers salt tolerance

doi:10.1007/s11103-007-9238-8

CORC > 中国农业科学院 > 作物科学研究所

	Overexpression of a putative maize calcineurin B-like protein in Arabidopsis confers salt tolerance
	Wang, Maoyan; Gu, Dan; Liu, Tingsong; Wang, Zhaoqiang; Guo, Xiying; Hou, Wei; Bai, Yunfeng; Chen, Xiaoping; Wang, Guoying
刊名	PLANT MOLECULAR BIOLOGY
	2007
卷号	65 期号:6 页码:733-746
关键词	CBL gene maize expression salt tolerance
ISSN号	0167-4412
DOI	10.1007/s11103-007-9238-8
通讯作者	Wang, Maoyan
英文摘要	The calcineurin B-like proteins (CBLs) represent a unique family of calcium sensors in plants. Although extensive studies and remarkable progress have been made in Arabidopsis (Arabidopsis thaliana) CBLs, their functions in other plant species are still quite limited. Here, we report the cloning and functional characterization of ZmCBL4, a novel CBL gene from maize (Zea mays). ZmCBL4 encodes a putative homolog of the Arabidopsis CBL4/SOS3 protein, with novel properties. ZmCBL4 has one copy in maize genome and harbors seven introns in its coding region. ZmCBL4 expressed differentially in various organs of the maize plants at a low level under normal condition, and its expression was regulated by NaCl, LiCl, ABA and PEG treatments. Expression of 35S::ZmCBL4 not only complemented the salt hypersensitivity in Arabidopsis sos3 mutant, but also enhanced the salt tolerance in Arabidopsis wild type at the germination and seedling stages. Moreover, the LiCl tolerance in all of the ZmCBL4-expressing lines increased more significantly as compared with the NaCl tolerance, and in consistent with this, it was found that the expression of Arabidopsis AtNHX8, a putative plasma membrane Li(+)/H(+) antiporter gene identified recently, was induced in these transgenic lines under LiCl stress. The ZmCBL4-expressing Arabidopsis lines accumulated less Na(+) and Li(+) as compared with the control plants. This study has identified a putative maize CBL gene which functions in the salt stress-elicited calcium signaling and thus in the tolerance to salinity.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000251174400004
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5649]
专题	作物科学研究所
作者单位	(1)Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China;(2)China Agr Univ, Natl Ctr Plant Gene Res, Beijing 100094, Peoples R China;(3)China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China;(4)Inner Mongolia Agr Univ, Coll Biotechnol, Hohhot, Peoples R China
推荐引用方式 GB/T 7714	Wang, Maoyan,Gu, Dan,Liu, Tingsong,et al. Overexpression of a putative maize calcineurin B-like protein in Arabidopsis confers salt tolerance[J]. PLANT MOLECULAR BIOLOGY,2007,65(6):733-746.
APA	Wang, Maoyan.,Gu, Dan.,Liu, Tingsong.,Wang, Zhaoqiang.,Guo, Xiying.,...&Wang, Guoying.(2007).Overexpression of a putative maize calcineurin B-like protein in Arabidopsis confers salt tolerance.PLANT MOLECULAR BIOLOGY,65(6),733-746.
MLA	Wang, Maoyan,et al."Overexpression of a putative maize calcineurin B-like protein in Arabidopsis confers salt tolerance".PLANT MOLECULAR BIOLOGY 65.6(2007):733-746.