Proteomics Identification of Differentially Expressed Leaf Proteins in Response to Setosphaeria turcica Infection in Resistant Maize

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	Proteomics Identification of Differentially Expressed Leaf Proteins in Response to Setosphaeria turcica Infection in Resistant Maize
	Zhang Xiaoli 1; Si Bingwen 2; Fan Chengming 1; Li Hongjie 1; Wang Xiaoming 1
刊名	Journal of Integrative Agriculture
	2014
卷号	13 期号:4 页码:789-803
关键词	maize Setosphaeria turcica Ht2 gene differential proteomics
ISSN号	2095-3119
英文摘要	Northern corn leaf blight (NCLB), caused by the heterothallic ascomycete fungus Setosphaeria turcica, is a destructive foliar disease of maize and represents a serious threat to maize production worldwide. A comparative proteomic study was conducted to explore the molecular mechanisms underlying the defense responses of the maize resistant line A619 Ht2 to S. turcica race 13. Leaf proteins were extracted from mock and S. turcica-infected leaves after inoculated for 72 h and analyzed for differentially expressed proteins using two-dimensional electrophoresis and mass spectrometry identification. 137 proteins showed reproducible differences in abundance by more than 2-fold at least, including 50 up-regulated proteins and 87 down-regulated proteins. 48 protein spots were successfully identified by MS analysis, which included 10 unique, 6 up-regulated, 20 down-regulated and 12 disappeared protein spots. These identified proteins were classified into 9 functional groups and involved in multiple functions, particularly in energy metabolism (46%), protein destination and storage (12%), and disease defense (18%). Some defense-related proteins were upregulated such as β-glucosidase, SOD, polyamines oxidase, HSC 70 and PPIases; while the expressions of photosynthesis- and metabolism-related proteins were down-regulated, by inoculation with S. turcica. The results indicated that a complex regulatory network was functioned in interaction between the resistant line A619 Ht2 and S. turcica. The resistance processes of A619 Ht2 mainly resided on directly releasing defense proteins, modulation of primary metabolism, affecting photosyntesis and carbohydrate metabolism.
学科主题	内科学 ; 铁路运输
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4955]
专题	作物科学研究所
作者单位	1.Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, 100081 2.Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, 100081
推荐引用方式 GB/T 7714	Zhang Xiaoli,Si Bingwen,Fan Chengming,et al. Proteomics Identification of Differentially Expressed Leaf Proteins in Response to Setosphaeria turcica Infection in Resistant Maize[J]. Journal of Integrative Agriculture,2014,13(4):789-803.
APA	Zhang Xiaoli,Si Bingwen,Fan Chengming,Li Hongjie,&Wang Xiaoming.(2014).Proteomics Identification of Differentially Expressed Leaf Proteins in Response to Setosphaeria turcica Infection in Resistant Maize.Journal of Integrative Agriculture,13(4),789-803.
MLA	Zhang Xiaoli,et al."Proteomics Identification of Differentially Expressed Leaf Proteins in Response to Setosphaeria turcica Infection in Resistant Maize".Journal of Integrative Agriculture 13.4(2014):789-803.