Development of Insertion and Deletion Markers based on Biparental Resequencing for Fine Mapping Seed Weight in Soybean

doi:10.3835/plantgenome2014.04.0014

CORC > 中国农业科学院 > 作物科学研究所

	Development of Insertion and Deletion Markers based on Biparental Resequencing for Fine Mapping Seed Weight in Soybean
	Li, Ying-hui 1; Liu, Bo 1; Reif, Jochen C.2; Liu, Yu-lin 1; Li, Hui-hui 1; Chang, Ru-zhen 1; Qiu, Li-juan 1
刊名	PLANT GENOME
	2014
卷号	7 期号:3 页码:-
ISSN号	1940-3372
DOI	10.3835/plantgenome2014.04.0014
通讯作者	Li, Ying-hui
英文摘要	As a complement to single nucleotide polymorphisms (SNPs) and simple sequence repeats (SSRs), biallelic insertions and deletions (InDels) represent powerful molecular markers with desirable features for filling the gap in current genetic linkage maps. In this study, 28,908 small InDel polymorphisms (1-5 base pair, bp) distributed genome-wide were identified and annotated by comparison of a whole-genome resequencing data set from two soybean [Glycine max (L.) Merr.] genotypes, cultivar Zhonghunag13 (ZH) and line Zhongpin03-5373 (ZP). The physical distribution of InDel polymorphisms in soybean genome was uneven, and matched closely with the distribution of previously annotated genes. The average density of InDel in the arm region was significantly higher than that in the pericentromeric region. The genomic regions that were fixed between the two elites were elucidated. With this information, five InDel markers within a putative quantitative trait locus (QTL) for seed weight (SW), qSW-17-2, were developed and used to genotype 254 recombinant inbred lines (RILs) derived from the cross of ZP x ZH. Adding these five InDel markers to previously used SNP and SSR markers facilitated the discovery of further recombination events allowing fine-mapping the QTL to a 0.5 Mbp region. Our study clearly underlines the high value of InDel markers for map-based cloning and marker-assisted selection in soybean.
学科主题	Plant Sciences ; Genetics & Heredity
语种	英语
出版者	CROP SCIENCE SOC AMER
WOS记录号	WOS:000345157300007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4815]
专题	作物科学研究所
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Germplasm Utilizat MOA, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Leibniz Inst Plant Genet & Crop Plant Res IPK, Dept Cytogenet & Genome Anal, D-06466 Gatersleben, Germany
推荐引用方式 GB/T 7714	Li, Ying-hui,Liu, Bo,Reif, Jochen C.,et al. Development of Insertion and Deletion Markers based on Biparental Resequencing for Fine Mapping Seed Weight in Soybean[J]. PLANT GENOME,2014,7(3):-.
APA	Li, Ying-hui.,Liu, Bo.,Reif, Jochen C..,Liu, Yu-lin.,Li, Hui-hui.,...&Qiu, Li-juan.(2014).Development of Insertion and Deletion Markers based on Biparental Resequencing for Fine Mapping Seed Weight in Soybean.PLANT GENOME,7(3),-.
MLA	Li, Ying-hui,et al."Development of Insertion and Deletion Markers based on Biparental Resequencing for Fine Mapping Seed Weight in Soybean".PLANT GENOME 7.3(2014):-.