Transcriptome-Wide Identification and Characterization of MicroRNAs from Castor Bean (Ricinus communis L.)

	Transcriptome-Wide Identification and Characterization of MicroRNAs from Castor Bean (Ricinus communis L.)
	Xu, Wei ; Cui, Qinghua ; Li, Fei ; Liu, Aizhong
刊名	PLOS ONE
	2013
卷号	8 期号:7 页码:-
ISSN号	1932-6203
中文摘要	Background: MicroRNAs (miRNAs) are endogenously encoded small RNAs that post-transcriptionally regulate gene expression and play essential roles in numerous developmental and physiological processes. Currently, little information on the transcriptome and tissue-specific expression of miRNAs is available in the model non-edible oilseed crop castor bean (Ricinus communis L.), one of the most important non-edible oilseed crops cultivated worldwide. Recent advances in sequencing technologies have allowed the identification of conserved and novel miRNAs in many plant species. Here, we used high-throughput sequencing technologies to identify and characterize the miRNAs in castor bean. Results: Five small RNA libraries were constructed for deep sequencing from root tips, leaves, developing seeds (at the initial stage, seed1; and at the fast oil accumulation stage, seed2) and endosperms in castor bean. High-throughput sequencing generated a large number of sequence reads of small RNAs in this study. In total, 86 conserved miRNAs were identified, including 63 known and 23 newly identified. Sixteen miRNA isoform variants in length were found from the conserved miRNAs of castor bean. MiRNAs displayed diverse organ-specific expression levels among five libraries. Combined with criteria for miRNA annotation and a RT-PCR approach, 72 novel miRNAs and their potential precursors were annotated and 20 miRNAs newly identified were validated. In addition, new target candidates for miRNAs newly identified in this study were proposed. Conclusions: The current study presents the first high-throughput small RNA sequencing study performed in castor bean to identify its miRNA population. It characterizes and increases the number of miRNAs and their isoforms identified in castor bean. The miRNA expression analysis provides a foundation for understanding castor bean miRNA organ-specific expression patterns. The present study offers an expanded picture of miRNAs for castor bean and other members in the family Euphorbiaceae.
学科主题	Science & Technology - Other Topics
原文出处	10.1371/journal.pone.0069995
语种	英语
公开日期	2017-06-12
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/10474]
专题	西双版纳热带植物园_其他
推荐引用方式 GB/T 7714	Xu, Wei,Cui, Qinghua,Li, Fei,et al. Transcriptome-Wide Identification and Characterization of MicroRNAs from Castor Bean (Ricinus communis L.)[J]. PLOS ONE,2013,8(7):-.
APA	Xu, Wei,Cui, Qinghua,Li, Fei,&Liu, Aizhong.(2013).Transcriptome-Wide Identification and Characterization of MicroRNAs from Castor Bean (Ricinus communis L.).PLOS ONE,8(7),-.
MLA	Xu, Wei,et al."Transcriptome-Wide Identification and Characterization of MicroRNAs from Castor Bean (Ricinus communis L.)".PLOS ONE 8.7(2013):-.