Coherent optical adaptive technique improves the spatial resolution of STED microscopy in thick samples | |
Yan, Wei1,2,3; Yang, Yanlong4; Tan, Yu2,3; Chen, Xun2,3; Li, Yang2,3; Qu, Junle1; Ye, Tong2,3,5 | |
刊名 | photonics research |
2017-06-01 | |
卷号 | 5期号:3页码:176-181 |
ISSN号 | 2327-9125 |
产权排序 | 4 |
英文摘要 | stimulated emission depletion (sted) microscopy is one of far-field optical microscopy techniques that can provide sub-diffraction spatial resolution. the spatial resolution of the sted microscopy is determined by the specially engineered beam profile of the depletion beam and its power. however, the beam profile of the depletion beam may be distorted due to aberrations of optical systems and inhomogeneity of a specimen's optical properties, resulting in a compromised spatial resolution. the situation gets deteriorated when thick samples are imaged. in the worst case, the severe distortion of the depletion beam profile may cause complete loss of the super-resolution effect no matter how much depletion power is applied to specimens. previously several adaptive optics approaches have been explored to compensate aberrations of systems and specimens. however, it is difficult to correct the complicated high-order optical aberrations of specimens. in this report, we demonstrate that the complicated distorted wavefront from a thick phantom sample can be measured by using the coherent optical adaptive technique. the full correction can effectively maintain and improve spatial resolution in imaging thick samples. (c) 2017 chinese laser press |
WOS标题词 | science & technology ; physical sciences |
类目[WOS] | optics |
研究领域[WOS] | optics |
关键词[WOS] | fluorescence microscopy ; confocal microscopy ; stimulated-emission ; aberrations ; nanoscopy ; limit ; deep |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000405990300006 |
内容类型 | 期刊论文 |
源URL | [http://ir.opt.ac.cn/handle/181661/29172] |
专题 | 西安光学精密机械研究所_瞬态光学技术国家重点实验室 |
作者单位 | 1.Shenzhen Univ, Coll Optoelect Engn, Minist Educ & Guangdong Prov, Key Lab Optoelect Devices & Syst, Shenzhen 518060, Peoples R China 2.Clemson Univ, Dept Bioengn, Clemson, SC 29634 USA 3.Clemson Univ, COMSET, Clemson, SC 29634 USA 4.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China 5.Med Univ South Carolina, Dept Regenerat Med & Cell Biol, Charleston, SC 29425 USA |
推荐引用方式 GB/T 7714 | Yan, Wei,Yang, Yanlong,Tan, Yu,et al. Coherent optical adaptive technique improves the spatial resolution of STED microscopy in thick samples[J]. photonics research,2017,5(3):176-181. |
APA | Yan, Wei.,Yang, Yanlong.,Tan, Yu.,Chen, Xun.,Li, Yang.,...&Ye, Tong.(2017).Coherent optical adaptive technique improves the spatial resolution of STED microscopy in thick samples.photonics research,5(3),176-181. |
MLA | Yan, Wei,et al."Coherent optical adaptive technique improves the spatial resolution of STED microscopy in thick samples".photonics research 5.3(2017):176-181. |
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