Chemical and biological characterization of a polysaccharide biological response modifier from Aloe vera L. var. chinensis (Haw.) Berg.

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	Chemical and biological characterization of a polysaccharide biological response modifier from Aloe vera L. var. chinensis (Haw.) Berg.
	M. Y. K. Leung ; C. Liu ; L. F. Zhu ; Hui YZ(惠永正) ; Yu B(俞飚) ; K. P. Fung
刊名	Glycobiology
	2004
卷号	14 期号:6 页码:501-510
ISSN号	0959-6658
其他题名	从Aloe vera L. var. chinensis (Haw.) Berg.提取的一种生理反应调节多糖: 化学和生物学表征
通讯作者	K. P. Fung
英文摘要	Three purified polysaccharide fractions designated as PAC-I, PAC-II, and PAC-III were prepared from Aloe vera L. var. chinensis (Haw.) Berg. by membrane fractionation and gel filtration HPLC. The polysaccharide fractions had molecular weights of 10,000 kDa, 1300 kDa, and 470 kDa, respectively. The major sugar residue in the polysaccharide fractions is mannose, which was found to be91.5% in PAC-I, 87.9% in PAC-II, and 53.7% in PAC-III. The protein contents in the polysaccharide fractions was undetectable. NMR study of PAC-I and PAC-II demonstrated the polysaccharides shared the same structure. The main skeletons of PAC-I and PAC-II are beta-(1-->4)-D linked mannose with acetylation at C-6 of manopyranosyl. The polysaccharide fractions stimulated peritoneal macrophages, splenic T and B cell proliferation, and activated these cells to secrete TNF-alpha, IL-1beta, INF-gamma, IL-2, and IL-6. The polysaccharides were nontoxic and exhibited potent indirect antitumor response in murine model. PAC-I, which had the highest mannose content and molecular weight, was found to be the most potent biological response modifier of the three fractions. Our results suggested that the potency of aloe polysaccharide fraction increases as mannose content and molecular weight of the polysaccharide fraction increase.
学科主题	生命有机化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1093/glycob/cwh050
语种	英语
WOS记录号	WOS:000221405900004
公开日期	2013-02-25
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/18987]
专题	上海有机化学研究所_生命有机化学国家重点实验室
推荐引用方式 GB/T 7714	M. Y. K. Leung,C. Liu,L. F. Zhu,et al. Chemical and biological characterization of a polysaccharide biological response modifier from Aloe vera L. var. chinensis (Haw.) Berg.[J]. Glycobiology,2004,14(6):501-510.
APA	M. Y. K. Leung,C. Liu,L. F. Zhu,惠永正,俞飚,&K. P. Fung.(2004).Chemical and biological characterization of a polysaccharide biological response modifier from Aloe vera L. var. chinensis (Haw.) Berg..Glycobiology,14(6),501-510.
MLA	M. Y. K. Leung,et al."Chemical and biological characterization of a polysaccharide biological response modifier from Aloe vera L. var. chinensis (Haw.) Berg.".Glycobiology 14.6(2004):501-510.