Solution structure and dynamics of human metallothionein-3 (MT-3) zinc

CORC > 上海有机化学研究所 > 中国科学院上海有机化学研究所 > 上海有机化学研究所

	Solution structure and dynamics of human metallothionein-3 (MT-3) zinc
	Wang H(王辉) ; Zhang Q(张琪) ; Cai B(蔡斌) ; Li HY(李红燕) ; Kong-Hung Sze ; Huang ZX(黄仲贤) ; Wu HM(吴厚铭) ; SUN HONGZHE
刊名	FEBS Lett.
	2006
卷号	580 期号:3 页码:795-800
ISSN号	0014-5793
其他题名	人金属硫蛋白 MT3的溶液结构和动力学
通讯作者	吴厚铭 ; SUN HONGZHE
英文摘要	Alzheimer's disease is characterized by progressive loss of neurons accompanied by the formation of intraneural neurofibrillary tangles and extracellular amyloid plaques. Human neuronal growth inhibitory factor, classified as metallothionein-3 (MT-3), was found to be related to the neurotrophic activity promoting cortical neuron survival and dendrite outgrowth in the cell culture studies. We have determined the solution structure of the a-domain of human MT-3 (residues 32-68) by multinuclear and multidimensional NMR spectroscopy in combination with the molecular dynamic simulated annealing approach. The human MT-3 shows two metal-thiolate clusters, one in the N-terminus (beta-domain) and one in the C-terminus (alpha-domain). The overall fold of the alpha-domain is similar to that of mouse MT-3. However, human MT-3 has a longer loop in the acidic hexapeptide insertion than that of mouse MT-3. Surprisingly, the backbone dynamics of the protein revealed that the beta-domain exhibits similar internal motion to the alpha-domain, although the N-terminal residues are more flexible. Our results may provide useful information for understanding the structure-function relationship of human MT-3. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
学科主题	生命有机化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1016/j.fcbsiet.2005.12.099
语种	英语
公开日期	2013-02-21
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/17373]
专题	上海有机化学研究所_上海有机化学研究所
推荐引用方式 GB/T 7714	Wang H,Zhang Q,Cai B,et al. Solution structure and dynamics of human metallothionein-3 (MT-3) zinc[J]. FEBS Lett.,2006,580(3):795-800.
APA	王辉.,张琪.,蔡斌.,李红燕.,Kong-Hung Sze.,...&SUN HONGZHE.(2006).Solution structure and dynamics of human metallothionein-3 (MT-3) zinc.FEBS Lett.,580(3),795-800.
MLA	王辉,et al."Solution structure and dynamics of human metallothionein-3 (MT-3) zinc".FEBS Lett. 580.3(2006):795-800.