题名 | 梅毒螺旋体特异性抗体胶体金免疫层析检测技术的构建及其应用; The building and application of Colloidal gold immunochromatographic assay in the detection for Treponema pallidum specific antibody |
作者 | 付左根 |
答辩日期 | 2012 ; 2012 |
导师 | 杨天赐 |
关键词 | 胶体金免疫层析技术 梅毒螺旋体 荧光密螺旋体抗体吸收试验 IgM IgG Colloidal Gold Immunochromatographic Assay Treponema pallidum Fluorescent Treponemal Antibody Absorption Test detection |
英文摘要 | 梅毒是由梅毒螺旋体(Treponemapallidum,Tp)感染引起的严重危害人类健康的性传播疾病。梅毒特异性抗体检测方法包括TPPA,ELISA,FTA-ABS及Western-blot等,其特异性均较高。一般地,TPPA,ELISA作为临床初筛,FTA-ABS和Western-blot作为确认试验。其中,FTA-ABS需要荧光显微镜,而且其结果判断需要训练有素和较丰富经验的专业人员才能完成。 本文应用梅毒特异性重组蛋白TPN17和TPN47建立梅毒螺旋体IgG/M胶体金免疫层析技术(ColloidalGold-immunochromatographyAssay,GICA),设定FTA-...; Syphilis is a kind of sexually transmitted disease caused by Treponema pallidum. The technologies for diagnosing syphilis whose specificity were higher, include TPPA, ELISA, FTA-ABS,Western-blot and so on. In general,TPPA and ELISA were used as clinical screening and FTA-ABS and Western-blot were the confirmatory tests for syphilis. The FTA-ABS test is based on immunofluorescent methods and the de...; 学位:理学硕士; 院系专业:医学院药学系_药理学; 学号:24520091153020 |
语种 | zh_CN |
出处 | http://210.34.4.13:8080/lunwen/detail.asp?serial=34886 |
内容类型 | 学位论文 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/52379] ![]() |
专题 | 医学院-学位论文 |
推荐引用方式 GB/T 7714 | 付左根. 梅毒螺旋体特异性抗体胶体金免疫层析检测技术的构建及其应用, The building and application of Colloidal gold immunochromatographic assay in the detection for Treponema pallidum specific antibody[D]. 2012, 2012. |
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