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	油茶粕抗皮肤细胞紫外损伤活性成分研究; Chemical component in camellia cake against ultraviolet rays radiation damage on skin cells
	Zhong SY(种姝伊) ; Shi XL(石欣蕾) ; Jiang FQ(蒋福全) ; Liu J(刘婕) ; Zhang XK(张晓坤) ; Chen QC(陈全成)
	2015-09-20
关键词	油茶粕 黄酮 皮肤细胞 抗紫外损伤 Camellia cake Flavonoids Skin cells Against UV damage
英文摘要	目的分离纯化油茶粕中抗皮肤细胞紫外损伤活性成分。方法硅胶色谱和高效液相反相制备色谱纯化油茶粕正丁醇提取物,WESTErn-blOTTIng法检测纯化物质对uV照射HA CA T皮肤细胞Jnk、P38激活以及MMP-9的表达的影响。结果分离得到一单体化合物,经鉴定为kAEMPfErOl 3-O-{β-d-gluCOPyrAnOSyl-(1->2)-[α-l-rHAMnOPyrAnOSyl-(1->6)]-β-d-gluCOPyrAnOSIdE},且该化合物能够抑制HA CA T皮肤细胞uV照射后Jnk、P38激活以及MMP-9表达。结论油茶粕含有抗皮肤细胞紫外损伤活性成分,值得进一步开发。; Objective To isolate the chemical component of camellia cake against ultraviolet rays radiation damage on skin cells.Methods Silica gel column chromatography and reverse phase preparative HPLC were used for the isolation of active compounds from the n-butanol extract of camellia cake.The effects of isolated compound on the activation of JNK and P38,and the protein expression of MMP-9 in UV irradiated Ha Ca J skin cells were determined by western blotting assay.Results A flavonoid glycoside was isolated from the camellia cake and identified as Kaempferol 3-O-{ β-D-glucopyranosyl-( 1-> 2)-[α-L-rhamnopyranosyl-( 1-> 6) ]-β-D-glucopyranoside} using NMR dada analysis.In addition,the isolated compound was showed to markedly inhibit the activation of JNK and P38,and MMP-9 protein expression in UV irradiated Ha Ca J skin cells.Conclusion The components in camellia cake might have significant activity on protecting skin from UV rays radiation damage,suggesting their potential development values.; 国家自然科学基金(No.81102332); 福建省厦门市科技计划项目(No.3502Z20123015)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/123614]
专题	药学院－已发表论文
推荐引用方式 GB/T 7714	种姝伊,石欣蕾,蒋福全,等. 油茶粕抗皮肤细胞紫外损伤活性成分研究, Chemical component in camellia cake against ultraviolet rays radiation damage on skin cells[J],2015.
APA	种姝伊,石欣蕾,蒋福全,刘婕,张晓坤,&陈全成.(2015).油茶粕抗皮肤细胞紫外损伤活性成分研究..
MLA	种姝伊,et al."油茶粕抗皮肤细胞紫外损伤活性成分研究".(2015).

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