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	苦参碱通过线粒体通路诱导HepG2细胞凋亡的机制研究; Matrine Induces Apoptosis of Hep G2 Cells via Mitochondrial Apoptotic Pathway
	周欢 ; 徐敏英 ; 雷宇 ; 梁凯 ; 郭晴 ; 胡天惠
	2014-11-15
关键词	苦参碱 Hep G2 线粒体通路 Matrine Hep G2 Mitochondrial apoptotic pathway
英文摘要	目的探讨苦参碱通过线粒体通路诱导HEP g2发生凋亡的机制。方法分别采用MTT法、PI染色法、流式细胞术检测苦参碱对肝癌HEP g2细胞的增殖抑制、周期调控、诱导凋亡作用;采用WESTErn blOT检测苦参碱诱导肝癌HEP g2细胞抑凋亡蛋白bId、bCl-2的表达量。结果苦参碱可抑制HEP g2细胞的增殖作用和诱导凋亡,呈时间和剂量依赖性,并可将HEP g2细胞周期阻滞在g0/g1期。苦参碱可引起线粒体膜电位崩溃,同时诱导bId、bCl-2表达下调,bAX表达上调。结论苦参碱通过调节细胞内bId、bCl-2和bAX蛋白的表达,经线粒体信号转导途径诱导人肝癌细胞系HEP g2发生凋亡。; Objective To explore the mechanism of matrine inducing apoptosis of Hep G2 cells via mitochondrial apoptotic pathway.Methods MTT assay,PI staining,flow cytometry were used to evaluate the anti-proliferation,cell cycle regulation,and inducing apoptosis effects of matrine on Hep G2 cells.Western Blot assay was used to detect the expression levels of anti-apoptotic protein Bid and Bcl-2 in Hep G2 cells.Results Matrine could inhibit the proliferation and induce the apoptosis of Hep G2 cells in a time- and dose-dependent manner,and the cell cycle of Hep G2 was arrested in G0 / G1 phase.Apoptosis of Hep G2 cells induced by matrine was associated with collapse of mitochondrial membrane potential,which made the Bid and Bcl-2 expression levels down-regulated but the Bax expression level up-regulated.Conclusion Matrine induces the apoptosis of human hepatoma cell line Hep G2 via the mitochondrial apoptotic pathway by regulating the expression levels of Bid,Bcl-2 and Bax proteins.; 国家自然科学基金资助项目(81172284); 福建省自然科学基金资助项目(2010J0615)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/123478]
专题	药学院－已发表论文
推荐引用方式 GB/T 7714	周欢,徐敏英,雷宇,等. 苦参碱通过线粒体通路诱导HepG2细胞凋亡的机制研究, Matrine Induces Apoptosis of Hep G2 Cells via Mitochondrial Apoptotic Pathway[J],2014.
APA	周欢,徐敏英,雷宇,梁凯,郭晴,&胡天惠.(2014).苦参碱通过线粒体通路诱导HepG2细胞凋亡的机制研究..
MLA	周欢,et al."苦参碱通过线粒体通路诱导HepG2细胞凋亡的机制研究".(2014).

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