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	Tat-TTA1-PEG共修饰明胶-硅氧烷纳米粒跨血脑屏障及对胶质瘤的靶向性研究; Research of Tat-TTA1-PEG modified gelatin-siloxane nanoparticles across blood-brain barrier and its targeting to glioma
	张龙 ; 胡洋 ; 林晓宁 ; 魏峰 ; 丰伟 ; 田新华
	2014-11-28
关键词	胶质瘤 纳米粒子 血脑屏障 靶向给药系统 glioma nanoparticles blood-brain barrier target drug delivery system
英文摘要	目的观察经TAT多肽,适配体TTA1,聚乙二醇(PEg)联合修饰的新型纳米载体明胶硅氧烷纳米粒子(gElATIn-SIlOXAnE nAnOPArTIClES,gS nPS)跨血脑屏障及靶向到达胶质瘤的能力。方法通过两步溶胶-凝胶法合成gS nPS,然后在其表面依次修饰上PEg、TTA1、TAT,并以荧光染料Cy5.5-nHS标记。建立胶质瘤裸鼠原位模型20~25只,分为空白对照组,gS nPS组,PEg-gS nPS组,TTA1-PEg-gS nPS组,TAT-TTA1-PEg-gS nPS组。尾静脉注射各种修饰的纳米粒子,观察其在大鼠体内分布情况;将裸鼠处死后取脑、肝、脾,观察纳米粒子在各器官的分布。结果活体成像及统计分析结果显示TTA1-TAT-PEg-gS nPS在脑部的荧光强于TAT-PEg-gS nPS、PEg-gS nPS、gS nPS组,且在肿瘤部位的荧光强于脑内其他部位,而在肝脏脾脏的荧光弱于其他3组。结论 TTA1-TAT-PEg-gS nPS能够逃逸内皮网状吞噬系统(rETICulOE EndOTHElIn SySTEM,rES)的吞噬,还能跨过血脑屏障,而且对胶质瘤具有靶向性,是一种潜在的胶质瘤治疗基因及药物的靶向载体。; Objective To observe the property of Tat-TTA1-PEGmodified gelatin-siloxane nanoparticles across the blood-brain barrier and glioma targeting.Methods GS NPs were prepared by two-step sol-gel method,and then labbled by Cy5.5-NHS.After that,PEG,aptamer TTA1 and Tat peptide were conjugate onto GS NPs respectively.Establish orthotopic implantation model of glioma(20 ~ 25),and they were divided into 5 groups:blank control group,GS NPs group,PEG-GS NPs group,TTA1-PEG-GS NPs group and Tat-TTA1-PEG-Gs group.GS NPs,PEG-GS NPs,TTA1-PEGGS NPs and Tat-TTA1-PEG-GS NPs were injected respectively into animal model,and their distribution via vivo imaging system were detected.To compare their distribution in major organ,the brain,liver and spleen were removed and visualized by vivo imaging system.Results Compared with other groups,Tat-TTA1-PEG-GS NPs injected mice had much fluorescent photons in brain,but less in liver and spleen.Conclusion Tat-TTA1-PEG-GS NPs could not only escape the capture of RES but also penetrate the blood-brain barrier and target to glioma.; 国家自然科学基金(30970733)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/123708]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	张龙,胡洋,林晓宁,等. Tat-TTA1-PEG共修饰明胶-硅氧烷纳米粒跨血脑屏障及对胶质瘤的靶向性研究, Research of Tat-TTA1-PEG modified gelatin-siloxane nanoparticles across blood-brain barrier and its targeting to glioma[J],2014.
APA	张龙,胡洋,林晓宁,魏峰,丰伟,&田新华.(2014).Tat-TTA1-PEG共修饰明胶-硅氧烷纳米粒跨血脑屏障及对胶质瘤的靶向性研究..
MLA	张龙,et al."Tat-TTA1-PEG共修饰明胶-硅氧烷纳米粒跨血脑屏障及对胶质瘤的靶向性研究".(2014).

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