Twist2 promotes self-renewal of liver cancer stem-like cells by regulating CD24

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	Twist2 promotes self-renewal of liver cancer stem-like cells by regulating CD24
	Liu, Allan Yi ; Cai, Yao ; Mao, Yubin ; Lin, Yancheng ; Zheng, Hong ; Wu, Tiantian ; Huang, Yangmei ; Fang, Xiaoguang ; Lin, Shuyong ; Feng, Qingzhao ; Huang, Zhengjie ; Yang, Tianci ; Luo, Qi ; Ouyang, Gaoliang ; Mao YB(毛宇彬) ; Lin SY(林舒勇) ; Huang ZJ(黄正接) ; Yang TC(杨天赐) ; Luo Q(罗琪)
刊名	http://dx.doi.org/10.1093/carcin/bgt364
	2014
关键词	EPITHELIAL-MESENCHYMAL TRANSITION TUMOR-INITIATING CELLS HEPATOCELLULAR-CARCINOMA METASTASIS GENE-EXPRESSION IDENTIFICATION TRANSCRIPTION INDUCTION PERIOSTIN PROFILES SURVIVAL
英文摘要	National Basic Research Program of China [2009CB941601, 2010CB732402]; National Nature Science Foundation of China [30871242, 30872515, 91029729, 31071302, 31171339, 81372841]; Programme of Introducing Talents of Discipline to Universities [B12001]; 111 Project of Education of China [B06016]; Fundamental Research Funds for the Central Universities [2010121095, 2011121062]; Scientific Fund for Distinguished Young Investigators of Fujian Province [2010J06013]; Twist2 is a highly conserved basic helix-loop-helix transcription factor that plays a critical role in embryogenesis. Recent evidence has revealed that aberrant Twist2 expression contributes to tumor progression; however, the role of Twist2 in human hepatocellular carcinoma (HCC) and its underlying mechanisms remain undefined. In this report, we demonstrate that Twist2 is overexpressed in human HCC tumors. We show that ectopic expression of Twist2 induces epithelialmesenchymal transition phenotypes, augments cell migration and invasion and colony-forming abilities in human HCC cells in vitro, and promotes tumor growth in vivo. Moreover, we found a higher percentage of CD24 liver cancer stem-like cells in Twist2-transduced HCC cells. Twist2-expressing cells exhibited an increased expression of stem cell markers Bmi-1, Sox2, CD24 and Nanog and an increased capacity for self-renewal. Knockdown of CD24 in HepG2/Twist2 cells decreased the levels of Sox2, pSTAT3 and Nanog, and reversed the cancer stem-like cell phenotypes induced by ectopic expression of Twist2. Furthermore, Twist2 regulated the CD24 expression by directly binding to the E-box region in CD24 promoter. Therefore, our data demonstrated that Twist2 augments liver cancer stem-like cell self-renewal in a CD24-dependent manner. Twist2CD24STAT3Nanog pathway may play a critical role in regulating liver cancer stem-like cell self-renewal. The identification of the Twist2-CD24 signaling pathway provides a potential therapeutic approach to target cancer stem cells in HCCs.
语种	英语
出版者	OXFORD UNIV PRESS
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93658]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Liu, Allan Yi,Cai, Yao,Mao, Yubin,et al. Twist2 promotes self-renewal of liver cancer stem-like cells by regulating CD24[J]. http://dx.doi.org/10.1093/carcin/bgt364,2014.
APA	Liu, Allan Yi.,Cai, Yao.,Mao, Yubin.,Lin, Yancheng.,Zheng, Hong.,...&罗琪.(2014).Twist2 promotes self-renewal of liver cancer stem-like cells by regulating CD24.http://dx.doi.org/10.1093/carcin/bgt364.
MLA	Liu, Allan Yi,et al."Twist2 promotes self-renewal of liver cancer stem-like cells by regulating CD24".http://dx.doi.org/10.1093/carcin/bgt364 (2014).