Preparation of decellularized and crosslinked artery patch for vascular tissue-engineering application

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	Preparation of decellularized and crosslinked artery patch for vascular tissue-engineering application
	Zhao, Yilin ; Zhang, Zhigang ; Wang, Jinling ; Yin, Ping ; Wang, Yu ; Yin, Zhenyu ; Zhou, Jianyin ; Xu, Gang ; Liu, Yun ; Deng, Zhigang ; Zhen, Maochuan ; Cui, Wugeng ; Liu, Zhongchen ; Zhao YL(赵一麟) ; Yin P(殷平) ; Yin ZY(尹震宇) ; Zhou JY(周剑寅) ; Deng ZG(邓志刚) ; Liu ZC(刘忠臣)
刊名	http://dx.doi.org/10.1007/s10856-011-4326-3
	2011-06
关键词	MESENCHYMAL STEM-CELLS MARROW-DERIVED CELLS HEART-VALVE IN-VIVO BOVINE PERICARDIUM SCAFFOLD LINKING CALCIFICATION COLLAGEN MATRIX
英文摘要	There is an urgent clinical need of tissue-engineering (TE) vascular grafts, so this study was for developing a fast and simple way of producing TE vascular scaffold. The TE vascular scaffold was prepared with pepsin, DNase and RNase enzymatic decellularization and crosslinked with 0.1, 1, 5% glutaraldehyde (GA), respectively. The samples were underwent analyses of burst pressure; suture strength; cytotoxicity; enzymatic degradation in vitro; degradation in vivo; rehydration; biocompatibilities detected with hematoxylin and eosin (H&E), scan electron microscope, immunohistochemistry both in vivo and in vitro; macrophage infiltration and calcification using Von Kossa staining. After being decellularized the scaffold had a complete removal of cellular components, an intact collagen structure. The burst pressure and suture strength were similar to native artery. 0.1% GA crosslinked scaffold showed less cytotoxicity than 1 and 5% GA groups (P < 0.05) and was resistance to enzymatic degradation in vitro. Once being implanted, 0.1% GA group was resistant to degradation and formed endothelium, smooth muscle and adventitia with few macrophages infiltration. However, there appeared calcification in implants compared with that in native artery. This study demonstrated that DVPs producing methods by enzymatic decellularizing and cross-linking with 0.1% GA could be used for clinical TE vascular graft manufacture.
语种	英语
出版者	J MATER SCI-MATER M
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93280]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Zhao, Yilin,Zhang, Zhigang,Wang, Jinling,et al. Preparation of decellularized and crosslinked artery patch for vascular tissue-engineering application[J]. http://dx.doi.org/10.1007/s10856-011-4326-3,2011.
APA	Zhao, Yilin.,Zhang, Zhigang.,Wang, Jinling.,Yin, Ping.,Wang, Yu.,...&刘忠臣.(2011).Preparation of decellularized and crosslinked artery patch for vascular tissue-engineering application.http://dx.doi.org/10.1007/s10856-011-4326-3.
MLA	Zhao, Yilin,et al."Preparation of decellularized and crosslinked artery patch for vascular tissue-engineering application".http://dx.doi.org/10.1007/s10856-011-4326-3 (2011).