Differential proteomic analysis of HL60 cells treated with secalonic acid F reveals caspase 3-induced cleavage of Rho GDP dissociation inhibitor 2 | |
Li, Ning ; Yi, Zhiwei ; Wang, Yuqiao ; Zhang, Qin ; Zhong, Tianhua ; Qiu, Yinkun ; Wu, Zhen ; Tang, Xixiang ; Qiu YK(邱鹰昆) | |
刊名 | http://dx.doi.org/10.3892/or.2012.2062 |
2012-12 | |
关键词 | FUNGUS PENICILLIUM SP INDUCED APOPTOSIS MYCOPHENOLIC-ACID DEEP-SEA D4-GDI LYMPHOCYTES SUBSTRATE SEDIMENT PROTEINS RHOGDI2 |
英文摘要 | Science and Technology project of Xiamen [3502Z20111051]; R&D Special Fund for Public Welfare Industry (Oceanography) [201005022-1, 201005032-1]; National Natural Science Foundation of China [81072549]; Secalonic acid F (SAF) has been previously identified, however, little is known about its cytotoxic activity and related cytotoxic mechanism. The aim of this study was to evaluate the cytotoxic activity of SAF isolated from a deep sea originated fungus Penicillium sp. F11 in HL60 cells and to analyze the differences in protein expression of HI.,60 cells treated with SAF. The CCK-8 assay and Annexin V-FLUOS/PI assay indicated that SAF displayed dose- and time-dependent inhibition of HL60 cell proliferation and induced apoptosis. Two-dimensional gel electrophoresis (2-DE) analysis of HL60 cells treated with SA F (4 mu g/ml) revealed 10 differentially expressed protein spots (P<0.05), 5 upregulated and 5 downregulated. Three spots (1 downregulated and 2 upregulated) were identified as Rho GDP dissociation inhibitor 2 (RhoGDI 2) proteins by MALDI-TOF MS. Western blotting further demonstrated the decreased abundance of full-length RhoGDI 2 together with the increased abundance of caspase 3-cleaved product of RhoGDI 2. The caspase 3 inhibitor Ac-DEVD-CHO could suppress the cytotoxic effect of SA F and significantly block the cleavage of RhoGDI 2. RhoGDI 2 is a cytosolic regulator of Rho GTPase and the caspase 3-cleaved product of RhoGDI 2 can advance progression of the apoptotic process. Our data showed that SAF may modulate RhoGDI 2 levels in HL60 cells, thereby potentially disrupting cell signaling pathways important for HL60 cell function. |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/71484] |
专题 | 医学院-已发表论文 |
推荐引用方式 GB/T 7714 | Li, Ning,Yi, Zhiwei,Wang, Yuqiao,et al. Differential proteomic analysis of HL60 cells treated with secalonic acid F reveals caspase 3-induced cleavage of Rho GDP dissociation inhibitor 2[J]. http://dx.doi.org/10.3892/or.2012.2062,2012. |
APA | Li, Ning.,Yi, Zhiwei.,Wang, Yuqiao.,Zhang, Qin.,Zhong, Tianhua.,...&邱鹰昆.(2012).Differential proteomic analysis of HL60 cells treated with secalonic acid F reveals caspase 3-induced cleavage of Rho GDP dissociation inhibitor 2.http://dx.doi.org/10.3892/or.2012.2062. |
MLA | Li, Ning,et al."Differential proteomic analysis of HL60 cells treated with secalonic acid F reveals caspase 3-induced cleavage of Rho GDP dissociation inhibitor 2".http://dx.doi.org/10.3892/or.2012.2062 (2012). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论