High-Resolution H-1 NMR Spectroscopy of Fish Muscle, Eggs and Small Whole Fish via Hadamard-Encoded Intermolecular Multiple-Quantum Coherence

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	High-Resolution H-1 NMR Spectroscopy of Fish Muscle, Eggs and Small Whole Fish via Hadamard-Encoded Intermolecular Multiple-Quantum Coherence
	Cai, Honghao ; Chen, Yushan ; Cui, Xiaohong ; Cai, Shuhui ; Chen, Zhong ; Cui XH(崔晓红) ; Cai SH(蔡淑惠) ; Chen Z(陈忠)
刊名	http://dx.doi.org/10.1371/journal.pone.0086422
	2014-01-17
关键词	SALMON SALMO-SALAR SPINNING NMR-SPECTROSCOPY INHOMOGENEOUS FIELDS PATTERN-RECOGNITION THYNNUS L. EXTRACTS LIVER PROFILE REGENERATION BOTTARGA
英文摘要	National Natural Science Foundation of China [11074209, 11174239, 21203155]; Fundamental Research Fund for the Central Universities [2010121008]; Background and Purpose: Nuclear magnetic resonance (NMR) spectroscopy has become an important technique for tissue studies. Since tissues are in semisolid-state, their high-resolution (HR) spectra cannot be obtained by conventional NMR spectroscopy. Because of this restriction, extraction and high-resolution magic angle spinning (HR MAS) are widely applied for HR NMR spectra of tissues. However, both of the methods are subject to limitations. In this study, the feasibility of HR H-1 NMR spectroscopy based on intermolecular multiple-quantum coherence (iMQC) technique is explored using fish muscle, fish eggs, and a whole fish as examples. Materials and Methods: Intact salmon muscle tissues, intact eggs from shishamo smelt and a whole fish (Siamese algae eater) are studied by using conventional 1D one-pulse sequence, Hadamard-encoded iMQC sequence, and HR MAS. Results: When we use the conventional 1D one-pulse sequence, hardly any useful spectral information can be obtained due to the severe field inhomogeneity. By contrast, HR NMR spectra can be obtained in a short period of time by using the Hadamard-encoded iMQC method without shimming. Most signals from fatty acids and small metabolites can be observed. Compared to HR MAS, the iMQC method is non-invasive, but the resolution and the sensitivity of resulting spectra are not as high as those of HR MAS spectra. Conclusion: Due to the immunity to field inhomogeneity, the iMQC technique can be a proper supplement to HR MAS, and it provides an alternative for the investigation in cases with field distortions and with samples unsuitable for spinning. The acquisition time of the proposed method is greatly reduced by introduction of the Hadamard-encoded technique, in comparison with that of conventional iMQC method.
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/92082]
专题	物理技术－已发表论文
推荐引用方式 GB/T 7714	Cai, Honghao,Chen, Yushan,Cui, Xiaohong,et al. High-Resolution H-1 NMR Spectroscopy of Fish Muscle, Eggs and Small Whole Fish via Hadamard-Encoded Intermolecular Multiple-Quantum Coherence[J]. http://dx.doi.org/10.1371/journal.pone.0086422,2014.
APA	Cai, Honghao.,Chen, Yushan.,Cui, Xiaohong.,Cai, Shuhui.,Chen, Zhong.,...&陈忠.(2014).High-Resolution H-1 NMR Spectroscopy of Fish Muscle, Eggs and Small Whole Fish via Hadamard-Encoded Intermolecular Multiple-Quantum Coherence.http://dx.doi.org/10.1371/journal.pone.0086422.
MLA	Cai, Honghao,et al."High-Resolution H-1 NMR Spectroscopy of Fish Muscle, Eggs and Small Whole Fish via Hadamard-Encoded Intermolecular Multiple-Quantum Coherence".http://dx.doi.org/10.1371/journal.pone.0086422 (2014).