High-Resolution Two-Dimensional J-Resolved NMR Spectroscopy for Biological Systems

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	High-Resolution Two-Dimensional J-Resolved NMR Spectroscopy for Biological Systems
	Huang, Yuqing ; Cal, Shuhui ; Zhang, Zhiyong ; Chen, Zhong ; Chen Z(陈忠)
刊名	http://dx.doi.org/10.1016/j.bpj.2014.03.022
	2014-05-06
关键词	MULTIPLE-QUANTUM COHERENCES INHOMOGENEOUS FIELDS IN-VIVO H-1-NMR SPECTROSCOPY BLOOD-PLASMA SPECTRA METABOLITES ENHANCEMENT SUPPRESSION ASSIGNMENT
英文摘要	National Natural Science Foundation of China [11205129, 11174239, 11275161]; Prior Research Field Fund for the Doctoral Program of Higher Education of China [20120121130003]; NMR spectroscopy is a principal tool in metabolomic studies and can, in theory, yield atom-level information critical for understanding biological systems. Nevertheless, NMR investigations on biological tissues generally have to contend with field inhomogeneities originating from variations in macroscopic magnetic susceptibility; these field inhomogeneities broaden spectral lines and thereby obscure metabolite signals. The congestion in one-dimensional NMR spectra of biological tissues often leads to ambiguities in metabolite identification and quantification. We propose an NMR approach based on intermolecular double-quantum coherences to recover high-resolution two-dimensional (2D) J-resolved spectra from inhomogeneous magnetic fields, such as those created by susceptibility variations in intact biological tissues. The proposed method makes it possible to acquire high-resolution 2D J-resolved spectra on intact biological samples without recourse to time-consuming shimming procedures or the use of specialized hardware, such as magic-angle-spinning probes. Separation of chemical shifts and J-couplings along two distinct dimensions is achieved, which reduces spectral crowding and increases metabolite specificity. Moreover, the apparent J coupling constants observed are magnified by a factor of 3, facilitating the accurate measurement of small J couplings, which is useful in metabolic analyses. Dramatically improved spectral resolution is demonstrated in our applications of the technique on pig brain tissues. The resulting spectra contain a wealth of chemical shift and J-coupling information that is invaluable for metabolite analyses. A spatially localized experiment applied on an intact fish (Crossocheilus siamensis) reveals the promise of the proposed method in in vivo metabolite studies. Moreover, the proposed method makes few demands on spectrometer hardware and therefore constitutes a convenient and effective manner for metabonomics study of biological systems.
语种	英语
出版者	CELL PRESS
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/92020]
专题	物理技术－已发表论文
推荐引用方式 GB/T 7714	Huang, Yuqing,Cal, Shuhui,Zhang, Zhiyong,et al. High-Resolution Two-Dimensional J-Resolved NMR Spectroscopy for Biological Systems[J]. http://dx.doi.org/10.1016/j.bpj.2014.03.022,2014.
APA	Huang, Yuqing,Cal, Shuhui,Zhang, Zhiyong,Chen, Zhong,&陈忠.(2014).High-Resolution Two-Dimensional J-Resolved NMR Spectroscopy for Biological Systems.http://dx.doi.org/10.1016/j.bpj.2014.03.022.
MLA	Huang, Yuqing,et al."High-Resolution Two-Dimensional J-Resolved NMR Spectroscopy for Biological Systems".http://dx.doi.org/10.1016/j.bpj.2014.03.022 (2014).