Spectroscopic studies on the interaction of Phacolysin and bovine serum albumin

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	Spectroscopic studies on the interaction of Phacolysin and bovine serum albumin
	Yu, Xianyong ; Liao, Zhixi ; Yao, Qing ; Liu, Heting ; Xie, Wenlin ; Yu XY(于贤勇)
刊名	http://dx.doi.org/10.1016/j.saa.2014.02.064
	2014-06-05
关键词	BETA-CYCLODEXTRIN SPECTRAL METHODS HYDROCHLORIDE FLUORESCENCE BINDING
英文摘要	Scientific Research Fund of Hunan Provincial Education Department [12K101, 13A028]; Hunan Provincial Natural Science Foundation of China [14JJ7049]; Science and Technology Project of Hunan Province [2013SK3161]; Opening Project of State Key Laboratory of Physical Chemistry of Solid Surfaces (Xiamen University) [201309]; Aid program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province; The interaction between Phacolysin (PCL) and bovine serum albumin (BSA) under imitated physiological conditions was investigated by spectroscopic (fluorescence, UV-Vis absorption and Circular dichroism) techniques. The experiments were conducted at different temperatures (294 K, 302 K, 306 K and 310 K) and the results showed that the PCL caused the fluorescence quenching of BSA through a static quenching procedure. The binding constant (K-a), binding sites (n) were obtained. The corresponding thermodynamic parameters (Delta H, Delta S and Delta G) of the interaction system were calculated at different temperatures. The results revealed that the binding process was spontaneous and the acting force between PCL and BSA were mainly hydrogen bonding and van der Waals forces. According to Forster non-radiation energy transfer theory, the binding distance between PCL and BSA was calculated to be 2.41 nm. What is more, both synchronous fluorescence and Circular dichroism spectra confirmed the interaction, which indicated the conformational changes of BSA. (C) 2014 Elsevier B.V. All rights reserved.
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/92009]
专题	物理技术－已发表论文
推荐引用方式 GB/T 7714	Yu, Xianyong,Liao, Zhixi,Yao, Qing,et al. Spectroscopic studies on the interaction of Phacolysin and bovine serum albumin[J]. http://dx.doi.org/10.1016/j.saa.2014.02.064,2014.
APA	Yu, Xianyong,Liao, Zhixi,Yao, Qing,Liu, Heting,Xie, Wenlin,&于贤勇.(2014).Spectroscopic studies on the interaction of Phacolysin and bovine serum albumin.http://dx.doi.org/10.1016/j.saa.2014.02.064.
MLA	Yu, Xianyong,et al."Spectroscopic studies on the interaction of Phacolysin and bovine serum albumin".http://dx.doi.org/10.1016/j.saa.2014.02.064 (2014).