Molecular cloning and expression of a cold-adapted lipase gene from an Antarctic deep sea psychrotrophic bacterium Pseudomonas sp 7323

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	Molecular cloning and expression of a cold-adapted lipase gene from an Antarctic deep sea psychrotrophic bacterium Pseudomonas sp 7323
	Zhang, Jin-wei ; Zeng, Run-ying ; Zhang JW(张剑文)
刊名	http://dx.doi.org/10.1007/s10126-008-9099-4
	2008-09
关键词	TERMINAL SIGNAL PEPTIDE GRAM-NEGATIVE BACTERIA ESCHERICHIA-COLI SERRATIA-MARCESCENS EXTRACELLULAR LIPASE PURIFICATION SECRETION ENZYMES OVERPRODUCTION SEQUENCE
英文摘要	A psychrotrophic bacterium producing a cold-adapted lipase was isolated from the deep-sea sediment of Prydz Bay, Antarctic and identified as a Pseudomonas strain. Determination of the nucleotide sequence of the gene encoding a lipase from Pseudomonas sp. 7323 (lipA) revealed that LipA is composed of 617 amino acid residues with a calculated molecular weight of 64,466 Da. LipA has a GXSXG motif, which is conserved in lipases/esterases and generally contains the active-site serine. The lipase purified from the Escherichia coli transformant (rLipA) by metal-chelating chromatography exhibited the same electrophoretic mobility as did the wild-type lipase (wLipA) purified from strain 7323, and both enzymes were quite similar in physicochemical properties. The optimal temperature and pH value for the lipases activity were 30 degrees C and 9.0, respectively. They were unstable at temperatures above 25 degrees C and only retained half of their highest activity after incubation at 60 degrees C for 5 min. These results indicated that the enzymes were typical alkaline cold-adapted enzymes. Both enzymes were particularly activated by Ca(2+). Additionally, the enzymes hydrolyzed p-nitrophenyl caprate and tributyrin at the highest velocity among the other p-nitrophenyl esters and triglycerides.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/66217]
专题	数学科学－已发表论文
推荐引用方式 GB/T 7714	Zhang, Jin-wei,Zeng, Run-ying,Zhang JW. Molecular cloning and expression of a cold-adapted lipase gene from an Antarctic deep sea psychrotrophic bacterium Pseudomonas sp 7323[J]. http://dx.doi.org/10.1007/s10126-008-9099-4,2008.
APA	Zhang, Jin-wei,Zeng, Run-ying,&张剑文.(2008).Molecular cloning and expression of a cold-adapted lipase gene from an Antarctic deep sea psychrotrophic bacterium Pseudomonas sp 7323.http://dx.doi.org/10.1007/s10126-008-9099-4.
MLA	Zhang, Jin-wei,et al."Molecular cloning and expression of a cold-adapted lipase gene from an Antarctic deep sea psychrotrophic bacterium Pseudomonas sp 7323".http://dx.doi.org/10.1007/s10126-008-9099-4 (2008).