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	硫酸脱氢表雄酮化学发光微粒子免疫法定量测定试剂的研制; Development of chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate
	黄明聪 ; 张晓琍 ; 林光华 ; 翁祖星 ; 孙旭东 ; 葛胜祥 ; 张军
	2015-09-18
关键词	硫酸脱氢表雄酮 化学发光微粒子免疫法 定量试剂盒 Dehydroepiandrosterone sulfate Chemiluminescent microparticle immunoassay Quantitative determination kit
英文摘要	目的研制硫酸脱氢表雄酮化学发光免疫定量检测试剂。方法利用硫酸脱氢表雄酮人工完全抗原免疫小鼠,通过杂交瘤技术制备特异性抗硫酸脱氢表雄酮单克隆抗体,采用竞争抑制法建立硫酸脱氢表雄酮化学发光免疫定量检测试剂。结果筛选获得了27株稳定分泌抗硫酸脱氢表雄酮的单克隆抗体细胞株,建立了化学发光微粒子免疫法定量测定硫酸脱氢表雄酮的试剂盒雏形,与雅培公司的硫酸脱氢表雄酮定量检测试剂在检测临床标本上的相关系数r达0.99以上。结论本研究为国产化硫酸脱氢表雄酮化学发光微粒子免疫法定量测定试剂盒的研发奠定了基础。; Objective To develop chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate.Methods Mice were immunized by dehydroepiandrosterone sulfate artificial complete antigen.Monoclonal antibodies against dehydroepiandrosterone sulfate were prepared by hybridoma technique.Chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate was developed based on competitive binding method.Results 27 monoclonal antibodies were obtained.Chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate prototype kit was established.The coefficient r of testing specimens with reagent from Abbott company was over 0.99.Conclusion Development of domestic chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate reagent was laid foundation by this study.; 国家高技术研究发展计划(863计划)(2011AA02A101)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/119327]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	黄明聪,张晓琍,林光华,等. 硫酸脱氢表雄酮化学发光微粒子免疫法定量测定试剂的研制, Development of chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate[J],2015.
APA	黄明聪.,张晓琍.,林光华.,翁祖星.,孙旭东.,...&张军.(2015).硫酸脱氢表雄酮化学发光微粒子免疫法定量测定试剂的研制..
MLA	黄明聪,et al."硫酸脱氢表雄酮化学发光微粒子免疫法定量测定试剂的研制".(2015).

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