Enhanced oxygen tolerance of hydrogenase from Klebsiella oxytoca HP1 by Gly-Cys exchanges nearby Fe-S clusters as biocatalysts in biofuel cells or hydrogen production

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	Enhanced oxygen tolerance of hydrogenase from Klebsiella oxytoca HP1 by Gly-Cys exchanges nearby Fe-S clusters as biocatalysts in biofuel cells or hydrogen production
	Huang, Gang-Feng ; Wu, Xiao-Bing ; Bai, Li-Ping ; Long, Min-Nan ; Chen, Qing-Xi ; Wu XB(邬小兵) ; Long MN(龙敏南) ; Chen QX(陈清西)
刊名	http://dx.doi.org/10.1016/j.ijhydene.2014.03.050
	2014
关键词	Amino acids Bacteria Biocatalysts Biofuels Biological fuel cells Enzymes Mutagenesis Oxygen Photolysis
英文摘要	Hydrogenase is the key point of H2-based biotechnology. However, the O2-sensitivity largely hinders its applications in biofuel cells and biological H2 production. Therefore, substantial breakthrough on understanding the molecular basis of O2-sensitivity and developing more O2-tolerant hydrogenases are urgently required. In this study, we found adding extra cysteines to the vicinity of the proximal Fe-S cluster to the NiFe active centre could largely enhance oxygen tolerance of hydrogen-evolving hydrogenase 3 from Klebsiella oxytoca HP1 (KoHyd3), through homologous sequence comparison and site-directed mutagenesis. Ratio of aerobic hydrogen yield to anaerobic hydrogen yield (RHH) of Gly47Cys (Gly47 was replaced with Cys47), Gly50Cys, Gly113Cys, Gly120Cys and Gly50Cys-Gly120Cys (double exchange) were increased by 46.99%, 42.15%, 59.19%, 44.74% and 78.72%, respectively, comparing with that of wild type. Moreover, TiO2-KoHyd3 (Gly47Cys, Gly50Cys, Gly113Cys, Gly120Cys and Gly50Cys-Gly120Cys) particles acted well in UV light-driven H2 production from water. These results revealed that extra cysteines nearby Fe-S clusters had significant effects on oxygen tolerance of KoHyd3. It also provided a promising way to produce O2-tolerant hydrogenase as biocatalysts in biofuel cells or H2 production by photolysis of water. ? 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
语种	英语
出版者	Elsevier Ltd
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90898]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Huang, Gang-Feng,Wu, Xiao-Bing,Bai, Li-Ping,et al. Enhanced oxygen tolerance of hydrogenase from Klebsiella oxytoca HP1 by Gly-Cys exchanges nearby Fe-S clusters as biocatalysts in biofuel cells or hydrogen production[J]. http://dx.doi.org/10.1016/j.ijhydene.2014.03.050,2014.
APA	Huang, Gang-Feng.,Wu, Xiao-Bing.,Bai, Li-Ping.,Long, Min-Nan.,Chen, Qing-Xi.,...&陈清西.(2014).Enhanced oxygen tolerance of hydrogenase from Klebsiella oxytoca HP1 by Gly-Cys exchanges nearby Fe-S clusters as biocatalysts in biofuel cells or hydrogen production.http://dx.doi.org/10.1016/j.ijhydene.2014.03.050.
MLA	Huang, Gang-Feng,et al."Enhanced oxygen tolerance of hydrogenase from Klebsiella oxytoca HP1 by Gly-Cys exchanges nearby Fe-S clusters as biocatalysts in biofuel cells or hydrogen production".http://dx.doi.org/10.1016/j.ijhydene.2014.03.050 (2014).