Dimeric structure of p300/CBP associated factor

CORC > 厦门大学 > 生命科学－已发表论文

	Dimeric structure of p300/CBP associated factor
	Shi, Shasha ; Lin, Juanyu ; Cai, Yongfei ; Yu, Jiao ; Hong, Haiyan ; Ji, Kunmei ; Downey, Jennifer S. ; Lu, Xiaodong ; Chen, Ruichuan ; Han, Jiahuai ; Han, Aidong ; Han JH(韩家淮) ; Han AD(韩爱东)
刊名	http://dx.doi.org/10.1186/1472-6807-14-2
	2014-01-14
关键词	HISTONE ACETYLTRANSFERASE DOMAIN HUMAN BRD2 BROMODOMAIN CRYSTAL-STRUCTURE SANT DOMAIN COENZYME-A P300/CBP-ASSOCIATED FACTOR SAGA COMPLEX DNA-DAMAGE ACETYLATION PCAF
英文摘要	National Science Foundation of China [31170685, 90919036, 30840027]; Project 985 [0660ZK1022]; Program 111 [B06016]; Background: p300/CBP associating factor (PCAF, also known as KAT2B for lysine acetyltransferase 2B) is a catalytic subunit of megadalton metazoan complex ATAC (Ada-Two-A containing complex) for acetylation of histones. However, relatively little is known about the regulation of the enzymatic activity of PCAF. Results: Here we present two dimeric structures of the PCAF acetyltransferase (HAT) domain. These dimerizations are mediated by either four-helical hydrophobic interactions or a beta-sheet extension. Our chemical cross-linking experiments in combined with site-directed mutagenesis demonstrated that the PCAF HAT domain mainly forms a dimer in solution through one of the observed interfaces. The results of maltose binding protein (MBP)-pulldown, co-immunoprecipitation and multiangle static light scattering experiments further indicated that PCAF dimeric state is detectable and may possibly exist in vivo. Conclusions: Taken together, our structural and biochemical studies indicate that PCAF appears to be a dimer in its functional ATAC complex.
语种	英语
出版者	BIOMED CENTRAL LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90846]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Shi, Shasha,Lin, Juanyu,Cai, Yongfei,et al. Dimeric structure of p300/CBP associated factor[J]. http://dx.doi.org/10.1186/1472-6807-14-2,2014.
APA	Shi, Shasha.,Lin, Juanyu.,Cai, Yongfei.,Yu, Jiao.,Hong, Haiyan.,...&韩爱东.(2014).Dimeric structure of p300/CBP associated factor.http://dx.doi.org/10.1186/1472-6807-14-2.
MLA	Shi, Shasha,et al."Dimeric structure of p300/CBP associated factor".http://dx.doi.org/10.1186/1472-6807-14-2 (2014).