Inhibitory kinetics of chlorocinnamic acids on mushroom tyrosinase

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	Inhibitory kinetics of chlorocinnamic acids on mushroom tyrosinase
	Hu, Yong-Hua ; Liu, Xuan ; Jia, Yu-Long ; Guo, Yun-Ji ; Wang, Qin ; Chen, Qing-Xi ; Wang Q(王勤) ; Chen QX(陈清西)
刊名	http://dx.doi.org/10.1016/j.jbiosc.2013.07.002
	2014
关键词	ANTIMICROBIAL ACTIVITIES CINNAMIC ACID ANTITYROSINASE DERIVATIVES
英文摘要	Natural Science Foundation of China [31071611, 31271952]; Science and Technology Foundation of Fujian Province [2010N5013]; Tyrosinase (EC 1.14.18.1) is the key enzyme of most food enzymatic oxidation. 'Tyrosinase inhibitors are important in food industry. In the present paper, 2-chlorcinnamic acid and 2,4-dichlorocinnamic acid were synthesized and the inhibitory kinetics on mushroom tyrosinase were investigated. The results showed that both compounds synthesized could inhibit tyrosinase activity. For monophenolase activity, both chlorocinnatnic acids could extended the lag time and decrease the steady-state activities, 2-chlorcinnamic acid extended the lag time just by 5%, and 2,4-dichlorcinnamic acid extended the lag time more than by 30.4%. For diphenolase activity, the IC50 values of 2-chlorcinnamic acid and 2,4dichlorocinnamic acid were determined to be 0.765 mM and 0.295 mM, respectively. The inhibition kinetics showed that 2-chlorcinnamic acid and 2,4-dichlorocinnamic acid displayed a reversible and uncompetitive mechanism. The inhibition constants were determined to be 0.348 mM and 0.159 mM, respectively. The research may supply the basis for designing new tyrosinase inhibitors.(C) 2013, The Society for Biotechnology, Japan. All rights
语种	英语
出版者	SOC BIOSCIENCE BIOENGINEERING JAPAN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90831]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Hu, Yong-Hua,Liu, Xuan,Jia, Yu-Long,et al. Inhibitory kinetics of chlorocinnamic acids on mushroom tyrosinase[J]. http://dx.doi.org/10.1016/j.jbiosc.2013.07.002,2014.
APA	Hu, Yong-Hua.,Liu, Xuan.,Jia, Yu-Long.,Guo, Yun-Ji.,Wang, Qin.,...&陈清西.(2014).Inhibitory kinetics of chlorocinnamic acids on mushroom tyrosinase.http://dx.doi.org/10.1016/j.jbiosc.2013.07.002.
MLA	Hu, Yong-Hua,et al."Inhibitory kinetics of chlorocinnamic acids on mushroom tyrosinase".http://dx.doi.org/10.1016/j.jbiosc.2013.07.002 (2014).