Structure Characterization and Anti-tyrosinase Mechanism of Polymeric Proanthocyanidins Fractionated from Kiwifruit Pericarp

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	Structure Characterization and Anti-tyrosinase Mechanism of Polymeric Proanthocyanidins Fractionated from Kiwifruit Pericarp
	Chai, Wei-Ming ; Shi, Yan ; Feng, Hui-Ling ; Xu, Lian ; Xiang, Zhi-Hao ; Gao, Yu-Sen ; Chen, Qing-Xi ; Shi Y(石艳) ; Chen QX(陈清西)
刊名	http://dx.doi.org/10.1021/jf501009v
	2014-07-09
关键词	ANTIOXIDANT ACTIVITY MUSHROOM TYROSINASE PHENOLIC-COMPOUNDS CONDENSED TANNINS MELANOMA-CELLS ANTITYROSINASE FLAVONOIDS PLANTS ASSAY MS
英文摘要	Natural Science Foundation of China [31071611, 31070522]; National Science Foundation for Fostering Talents in Basic Research of the National Natural Science Foundation of China [J1310027/J0106]; Science and Technology Foundation of Fujian Province [2010N5013]; National Natural Science Fund of China [31371988]; To provide information on the structure, activity, and structure-activity relationship of kiwifruit (Actinidia chinensis) pericarp proanthocyanidins (PAs), they were separated into three fractions. These fractions were further identified by MALDI-TOF MS and HPLC-ESI-MS methods. Spectra results revealed that they are complex mixtures of B-type propelargonidins, procyanidins, procyanidins gallate, and prodelphinidins. Enzymatic activity analysis showed that these compounds strongly inhibit the activity of tyrosinase, indicating that they are reversible and mixed-type inhibitors of the enzyme. The results obtained from fluorescence quenching showed PAs inhibit the enzyme activity by interacting with substrate and enzyme. This study confirmed that the mean degree of polymerization (mDP) of PAs produces a positive effect on their anti-tyrosinase activity. In addition, the antioxidant analysis indicated that PAs possess potent antioxidant activity. These conclusions mean kiwifruit pericarp PAs may be explored as insecticides, food preservatives, and cosmetic additives.
语种	英语
出版者	AMER CHEMICAL SOC
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90762]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Chai, Wei-Ming,Shi, Yan,Feng, Hui-Ling,et al. Structure Characterization and Anti-tyrosinase Mechanism of Polymeric Proanthocyanidins Fractionated from Kiwifruit Pericarp[J]. http://dx.doi.org/10.1021/jf501009v,2014.
APA	Chai, Wei-Ming.,Shi, Yan.,Feng, Hui-Ling.,Xu, Lian.,Xiang, Zhi-Hao.,...&陈清西.(2014).Structure Characterization and Anti-tyrosinase Mechanism of Polymeric Proanthocyanidins Fractionated from Kiwifruit Pericarp.http://dx.doi.org/10.1021/jf501009v.
MLA	Chai, Wei-Ming,et al."Structure Characterization and Anti-tyrosinase Mechanism of Polymeric Proanthocyanidins Fractionated from Kiwifruit Pericarp".http://dx.doi.org/10.1021/jf501009v (2014).