Inhibitory Effects of Propyl Gallate on Tyrosinase and Its Application in Controlling Pericarp Browning of Harvested Longan Fruits

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	Inhibitory Effects of Propyl Gallate on Tyrosinase and Its Application in Controlling Pericarp Browning of Harvested Longan Fruits
	Lin, Yi-Fen ; Hu, Yong-Hua ; Lin, He-Tong ; Liu, Xuan ; Chen, Yi-Hui ; Zhang, Shen ; Chen, Qing-Xi ; Chen QX(陈清西)
刊名	http://dx.doi.org/10.1021/jf305481h
	2013
关键词	MUSHROOM TYROSINASE MELANIN FORMATION LITCHI FRUIT QUALITY ACID THIOSEMICARBAZONES KINETICS OXIDASE STORAGE DECAY
英文摘要	National Natural Science Foundation of China [30972070, 311711776, 31071611]; Specialized Research Fund for the Doctoral Program of Higher Education of China [20093515110011]; Tyrosinase (EC 1.14.18.1), also known as polyphenol oxidase (PPO), is a key enzyme in pigment biosynthesis of organisms. The inhibitory effects of propyl gallate on the activity of mushroom tyrosinase and effects of propyl gallate on pericarp browning of harvested longan fruits in relation to phenolic metabolism were investigated The results showed that propyl gallate could potently inhibit diphenolase activity of tyrosinase. The inhibitor concentration leading to 50% activity lost (IC50) was determined to be 0.685 mM. Kinetic analyses showed that propyl gallate was a reversible and mixed type inhibitor on this enzyme. The inhibition constants (K-IS and K-I) were determined to be 2.135 and 0.661 mM, respectively. Furthermore, the results also showed that propyl gallate treatment inhibited activities of PPO and POD in pericarp of harvested longan fruits, and maintained higher contents of total phenol and flavonoid of longan pericarp. Moreover, propyl gallate treatment also delayed the increases of browning index and browning degree in pericarp of harvested longan fruits. Therefore, application of propyl gallate may be a promising method for inhibiting tyrosinase activity, controlling pericarp browning, and extending shelf life of harvested longan fruits.
语种	英语
出版者	AMER CHEMICAL SOC
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90742]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Lin, Yi-Fen,Hu, Yong-Hua,Lin, He-Tong,et al. Inhibitory Effects of Propyl Gallate on Tyrosinase and Its Application in Controlling Pericarp Browning of Harvested Longan Fruits[J]. http://dx.doi.org/10.1021/jf305481h,2013.
APA	Lin, Yi-Fen.,Hu, Yong-Hua.,Lin, He-Tong.,Liu, Xuan.,Chen, Yi-Hui.,...&陈清西.(2013).Inhibitory Effects of Propyl Gallate on Tyrosinase and Its Application in Controlling Pericarp Browning of Harvested Longan Fruits.http://dx.doi.org/10.1021/jf305481h.
MLA	Lin, Yi-Fen,et al."Inhibitory Effects of Propyl Gallate on Tyrosinase and Its Application in Controlling Pericarp Browning of Harvested Longan Fruits".http://dx.doi.org/10.1021/jf305481h (2013).