Protective Roles of Gadd45 and MDM2 in Blueberry Anthocyanins Mediated DNA Repair of Fragmented and Non-Fragmented DNA Damage in UV-Irradiated HepG2 Cells

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	Protective Roles of Gadd45 and MDM2 in Blueberry Anthocyanins Mediated DNA Repair of Fragmented and Non-Fragmented DNA Damage in UV-Irradiated HepG2 Cells
	Liu, Wei ; Lu, Xiangyi ; He, Guangyang ; Gao, Xiang ; Xu, Maonian ; Zhang, Jingkai ; Li, Meiling ; Wang, Lifeng ; Li, Zhenjing ; Wang, Likui ; Luo, Cheng ; He GY(贺光阳)
刊名	http://dx.doi.org/10.3390/ijms141121447
	2013
关键词	CYCLE CHECKPOINTS OXIDATIVE STRESS P53 PHOSPHORYLATION IDENTIFICATION PROGRESSION EXTRACTION APOPTOSIS CANCER ATM
英文摘要	Tianjin city government; Growth Arrest and DNA Damage-inducible 45 (Gadd45) and MDM2 proteins, together with p21 and p53, play important roles in cell cycle checkpoints, DNA repair, and genome integrity maintenance. Gadd45 and MDM2 were activated and transcribed instantly by UV irradiation, whereas blueberry anthocyanins (BA) decreased the gene and protein expression levels in HepG2 cells for up to 24 h, and gradually restored the UV-induced fragmented and non-fragmented DNA damage of the nucleus at a time point of 12 h. Nevertheless, UV-irradiated HepG2 cell arrests occurred mainly in the G1 phase, which indicated G1 as a checkpoint. The proteins, p21 and p53, retain cellular integrity, suppressing the oncogenic transformation by interruption of the G1 phase of the cellular cycle, giving time for repairing the damage to DNA, or apoptosis induction if the damage is too severe to be repaired, while MDM2 and Gadd45 concomitantly ensure the presence of p53 and p21. Thus, we conclude that repair, together with Gadd45 and MDM2 genes, were involved in light and dark reaction mechanisms, however, BA could interfere and assist the repair through restoration, although further studies of the complex of the gene cascades triggered and responded to in BA-assisted DNA repair are needed.
语种	英语
出版者	MDPI AG
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90655]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Liu, Wei,Lu, Xiangyi,He, Guangyang,et al. Protective Roles of Gadd45 and MDM2 in Blueberry Anthocyanins Mediated DNA Repair of Fragmented and Non-Fragmented DNA Damage in UV-Irradiated HepG2 Cells[J]. http://dx.doi.org/10.3390/ijms141121447,2013.
APA	Liu, Wei.,Lu, Xiangyi.,He, Guangyang.,Gao, Xiang.,Xu, Maonian.,...&贺光阳.(2013).Protective Roles of Gadd45 and MDM2 in Blueberry Anthocyanins Mediated DNA Repair of Fragmented and Non-Fragmented DNA Damage in UV-Irradiated HepG2 Cells.http://dx.doi.org/10.3390/ijms141121447.
MLA	Liu, Wei,et al."Protective Roles of Gadd45 and MDM2 in Blueberry Anthocyanins Mediated DNA Repair of Fragmented and Non-Fragmented DNA Damage in UV-Irradiated HepG2 Cells".http://dx.doi.org/10.3390/ijms141121447 (2013).